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首页> 美国卫生研究院文献>Asian-Australasian Journal of Animal Sciences >Effect of Glycosaminoglycans on In vitro Fertilizing Ability and In vitro Developmental Potential of Bovine Embryos
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Effect of Glycosaminoglycans on In vitro Fertilizing Ability and In vitro Developmental Potential of Bovine Embryos

机译：糖胺聚糖对牛胚胎体外受精能力和体外发育潜能的影响

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The glycosaminoglycans (GAGs) present in the female reproductive tract promote sperm capacitation. When bovine sperm were exposed to 10 μg/ml of one of four GAGs (Chondroitin sulfate, CS; Dermatan sulfate, DS; Hyaluronic acid, HA; Heparin, HP) for 5 h, the total motility (TM), straight-line velocity (VSL), and curvilinear velocity (VCL) were higher in the HP- or HA-treated sperm, relative to control and CS- or DS-treated sperm. HP and HA treatments increased the levels of capacitated and acrosome-reacted sperm over time, compared to other treatment groups (p<0.05). In addition, sperm exposed to HP or HA for 1 h before IVF exhibited significantly improved fertilizing ability, as assessed by 2 pronucleus (PN) formation and cleavage rates at d 2. Exposure to these GAGs also enhanced in vitro embryo development rates and embryo quality, and increased the ICM and total blastocyst cell numbers at d 8 after IVF (p<0.05). A real-time PCR analysis showed that the expression levels of pluripotency (Oct 4), cell growth (Glut 5), and anti-apoptosis (Bax inhibitor) genes were significantly higher in embryos derived from HA- or HP-treated sperm than in control or other treatment groups, while pro-apoptotic gene expression (caspase-3) was significantly lower in all GAG treatment groups (p<0.05). These results demonstrated that exposure of bovine sperm to HP or HA positively correlates with in vitro fertilizing ability, in vitro embryo developmental potential, and embryonic gene expression.
机译：女性生殖道中存在的糖胺聚糖（GAG）促进精子获能。当将牛精子暴露于10μg/ ml的四种GAG（硫酸软骨素，CS;硫酸皮肤素，DS;透明质酸，HA;肝素，HP）中的一种时，其总运动度（TM），直线速度为5小时相对于对照和CS或DS处理的精子，HP或HA处理的精子的（VSL）和曲线速度（VCL）更高。与其他治疗组相比，HP和HA治疗随时间的推移会增加获能和顶体反应的精子水平（p <0.05）。此外，IVF前暴露于HP或HA 1 h的精子表现出显着提高的受精能力，这是通过第2天的2个前核（PN）形成和卵裂率评估的。 ，并且在IVF后第8天增加了ICM和总胚泡细胞数（p <0.05）。实时PCR分析显示，用HA或HP处理的精子衍生的胚胎中，多能性（10月4日），细胞生长（Glut 5）和抗凋亡（Bax抑制剂）基因的表达水平显着更高。对照组或其他治疗组，而促凋亡基因表达（caspase-3）在所有GAG治疗组中均显着降低（p <0.05）。这些结果表明，牛精子暴露于HP或HA与体外受精能力，体外胚胎发育潜能和胚胎基因表达呈正相关。

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