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首页> 美国卫生研究院文献>Asian-Australasian Journal of Animal Sciences >Optimal Cultivation Time for Yeast and Lactic Acid Bacteria in Fermented Milk and Effects of Fermented Soybean Meal on Rumen Degradability Using Nylon Bag Technique
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Optimal Cultivation Time for Yeast and Lactic Acid Bacteria in Fermented Milk and Effects of Fermented Soybean Meal on Rumen Degradability Using Nylon Bag Technique

机译：尼龙袋技术在发酵乳中酵母和乳酸菌的最佳培养时间及发酵豆粕对瘤胃降解的影响

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The objectives of this study were to determine an optimal cultivation time for populations of yeast and lactic acid bacteria (LAB) co-cultured in fermented milk and effects of soybean meal fermented milk (SBMFM) supplementation on rumen degradability in beef cattle using nylon bag technique. The study on an optimal cultivation time for yeast and LAB growth in fermented milk was determined at 0, 4, 8, 24, 48, 72, and 96 h post-cultivation. After fermenting for 4 days, an optimal cultivation time of yeast and LAB in fermented milk was selected and used for making the SBMFM product to study nylon bag technique. Two ruminal fistulated beef cattle (410±10 kg) were used to study on the effect of SBMFM supplementation (0%, 3%, and 5% of total concentrate substrate) on rumen degradability using in situ method at incubation times of 0, 2, 4, 6, 12, 24, 48, and 72 h according to a Completely randomized design. The results revealed that the highest yeast and LAB population culture in fermented milk was found at 72 h-post cultivation. From in situ study, the soluble fractions at time zero (a), potential degradability (a+b) and effective degradability of dry matter (EDDM) linearly (p<0.01) increased with the increasing supplemental levels and the highest was in the 5% SBMFM supplemented group. However, there was no effect of SBMFM supplement on insoluble degradability fractions (b) and rate of degradation (c). In conclusion, the optimal fermented time for fermented milk with yeast and LAB was at 72 h-post cultivation and supplementation of SBMFM at 5% of total concentrate substrate could improve rumen degradability of beef cattle. However, further research on effect of SBMFM on rumen ecology and production performance in meat and milk should be conducted using in vivo both digestion and feeding trials.
机译：这项研究的目的是确定使用尼龙袋技术在发酵乳中共培养的酵母和乳酸菌（LAB）群体的最佳培养时间，以及补充豆粕发酵乳（SBMFM）对肉牛瘤胃降解性的影响。 。在发酵后0、4、8、24、48、72和96 h确定了酵母和LAB在发酵乳中最佳生长时间的研究。发酵4天后，选择酵母和LAB在发酵乳中的最佳培养时间，并用于制备SBMFM产品，以研究尼龙袋技术。使用两只瘤胃瘘的牛（410±10 kg），在0、2的孵育时间下，采用原位方法研究了补充SBMFM（占总浓缩基质的0％，3％和5％）对瘤胃降解性的影响。 4、6、12、24、48和72小时根据完全随机设计进行。结果表明，发酵后72小时发现发酵乳中酵母和乳酸菌种群的最高培养。根据原位研究，零添加量（a），潜在降解性（a + b）和干物质有效降解性（EDDM）的可溶性部分随补充水平的增加呈线性增加（p <0.01），最高的是5 ％SBMFM补充组。但是，补充SBMFM对不溶性降解率（b）和降解速率（c）没有影响。综上所述，酵母和乳酸菌发酵乳的最佳发酵时间是培养后72 h，添加SBMFM浓度为总精料的5％可以改善肉牛的瘤胃降解性。但是，应该使用体内消化和饲喂试验对SBMFM对瘤胃生态和肉和奶生产性能的影响进行进一步研究。

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