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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Microbial Metabolism and Dynamic Changes in the Electrical Conductivity of Soil Solutions: a Method for Detecting Extraterrestrial Life
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Microbial Metabolism and Dynamic Changes in the Electrical Conductivity of Soil Solutions: a Method for Detecting Extraterrestrial Life

机译：微生物代谢和土壤溶液电导率的动态变化：一种检测外星生命的方法

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摘要

The addition of 0.5% glucose solutions to 12 different air-dried soils always resulted in increased electrical conductivity and water-soluble Ca and Mg in the soil solutions. The kinetics and magnitude of these changes for at least two and usually all three of these parameters over a 14-day period were clearly distinguishable from the changes in heat-sterilized controls or unsterilized controls without added glucose. In general, maximal values were achieved more rapidly under aerobic than anaerobic incubation. Some soils (less than half) also showed significant increases in water-soluble Na or K when compared with the controls. The 12 different soils studied represented four general soil groups: I, leached acid upland soils; II, saline alkaline soils; III, nonsaline neutral soils; and IV, high organic soils. Viable counts ranged from 104 to 107 per cm3 of air-dried soil. Glucose metabolism by the indigenous soil microbiota was always accompanied by a significant decrease in the pH of soil solutions, but not necessarily by an increase in the viable count. The feasibility of using electrical conductivity and water-soluble Ca and Mg measurements to detect metabolic activity, either alone or in conjunction with other life detection techniques, is discussed.
机译：在12种不同的风干土壤中添加0.5％的葡萄糖溶液总是会增加土壤溶液中的电导率以及水溶性Ca和Mg。在14天的时间段内，至少有两个参数（通常是所有三个参数）的这些变化的动力学和大小可以与不添加葡萄糖的热灭菌对照或未灭菌对照的变化区分开来。通常，有氧培养比无氧培养更快地达到最大值。与对照相比，某些土壤（少于一半）的水溶性Na或K也显着增加。所研究的12种不同的土壤代表了四个一般的土壤组：I，淋溶的酸性高地土壤；二，盐碱土；三，非盐类中性土壤；第四，高有机土壤。每厘米 3 风干土壤中的活菌数范围为10 4 到10 7 。土著土壤微生物群的葡萄糖代谢总是伴随着土壤溶液pH值的显着降低，但不一定伴随着活菌数的增加。讨论了单独使用或与其他生命检测技术结合使用电导率和水溶性Ca和Mg测量来检测代谢活性的可行性。

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