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Bicarbonate Uptake by Nitrifiers: Effects of Growth Rate pH Substrate Concentration and Metabolic Inhibitors

机译:硝化剂吸收碳酸氢盐:增长率pH底物浓度和代谢抑制剂的影响

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摘要

The ratios of bicarbonate uptake to substrate oxidation were measured for three genera of nitrifying bacteria. The ratios for the two ammonium oxidizers tested were essentially the same; 0.0863 ± 0.0055 and 0.0868 ± 0.0091 μmol of bicarbonate were taken up per umol ammonium oxidized for Nitrosomonas europaea and a Nitrosospira strain, respectively. For Nitrobacter sp., a ratio of 0.0236 ± 0.0013 μmol of bicarbonate taken up per umol of nitrite oxidized was obtained. Cells were grown in substrate-limited continuous culture and in batch culture, with generation times ranging between 16 and 189 h for the ammonium oxidizers and 18 and 69 h for Nitrobacter sp. All ratios appeared to be independent of growth rates and pH. However, short-term changes in substrate concentration and certain metabolic inhibitors significantly changed the efficiency of bicarbonate uptake. The significance of these results to the application of the nitrapyrin-sensitive bicarbonate uptake method for measuring nitrification rates in natural samples is discussed.
机译:测量了三属硝化细菌的碳酸氢根吸收与底物氧化的比率。所测试的两种铵氧化剂的比例基本相同。对于欧洲亚硝化单胞菌和亚硝基螺菌菌株,每个氧化的umol铵分别吸收0.0863±0.0055和0.0868±0.0091μmol的碳酸氢盐。对于硝化细菌,每摩尔被氧化亚硝酸酯吸收的碳酸氢盐比例为0.0236±0.0013μmol。细胞在受底物限制的连续培养和分批培养中生长,铵氧化剂的生成时间在16至189小时之间,硝化细菌sp的生成时间在18至69小时之间。所有比率似乎均与生长速率和pH无关。但是,底物浓度和某些代谢抑制剂的短期变化显着改变了碳酸氢盐吸收的效率。讨论了这些结果对应用硝普林敏感的碳酸氢盐吸收方法测量自然样品中硝化率的意义。

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