首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Polyethylene glycol-induced internalization of bacteria into fungal protoplasts: electron microscopic study and optimization of experimental conditions.

【2h】

Polyethylene glycol-induced internalization of bacteria into fungal protoplasts: electron microscopic study and optimization of experimental conditions.

机译：聚乙二醇诱导的细菌内化为真菌原生质体：电子显微镜研究和实验条件的优化。

代理获取

本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。

页面导航

摘要
著录项
相似文献
相关主题

摘要

We studied the mechanism of internalization of Escherichia coli into Saccharomyces cerevisiae induced by polyethylene glycol (PEG) and optimized the experimental conditions. Transmission electron microscope studies revealed that the principal factor involved in the internalization was the degree of cell aggregation attained. Internalization occurred mainly by an endocytosis-like mechanism and took place during the elimination of PEG. The optimum conditions were to treat a mixed pellet of both microorganisms with 15% PEG and then gradually dilute the polymer. The same conditions were applied to E. coli and Aspergillus nidulans, with similar results.

机译：我们研究了由聚乙二醇（PEG）诱导的大肠杆菌内化为酿酒酵母的机理，并优化了实验条件。透射电子显微镜研究表明，参与内在化的主要因素是所达到的细胞聚集程度。内部化主要通过内吞作用样机制发生，并且发生在PEG消除过程中。最佳条件是用15％PEG处理两种微生物的混合沉淀，然后逐渐稀释聚合物。将相同条件应用于大肠杆菌和构巢曲霉，结果相似。

著录项

期刊名称 Applied and Environmental Microbiology
作者
I Guerra-Tschuschke; I Martín; M T González;
展开▼
作者单位

展开▼
年(卷),期 1991(57),5
年度 1991
页码 1516–1522
总页数 7
原文格式 PDF
正文语种
中图分类应用微生物学;微生物学;
关键词

相似文献

外文文献
中文文献
专利

1. Polyethylene glycol-induced internalization of bacteria into fungal protoplasts: electron microscopic study and optimization of experimental conditions. [J] . I Guerra-Tschuschke, I Martín, M T González Applied and Environmental Microbiology . 1991,第5期

机译：聚乙二醇诱导的细菌内化为真菌原生质体：电子显微镜研究和实验条件的优化。
2. POLYETHYLENE GLYCOL-INDUCED FUSION OF YEAST AND FILAMENTOUS FUNGI PROTOPLASTS : ELECTRON MICROSCOPIC STUDY [J] . NIVEDITA SINGH, U. S. BAGDE Asian Journal of Microbiology, Biotechnology and Environmental Science . 2009,第3期

机译：聚乙烯醇诱导的酵母融合和丝状真菌原生质体：电子显微镜研究
3. Effect of Bacillus subtilis BsuM restriction-modification on plasmid transfer by polyethylene glycol-induced protoplast fusion [J] . Maehara T., Itaya M., Ogura M., FEMS Microbiology Letters . 2011,第1期

机译：枯草芽孢杆菌BsuM限制性修饰对聚乙二醇诱导原生质体融合质粒转移的影响
4. Experimental study on improving nitrogen removal efficiency of denitrify bacteria by protoplast fusion [C] . Wen-jiao SANG, Bei-ping ZHANG, Zhen LIU, The 2nd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2008)(第二届生物信息与生物医学工程国际会议）论文集 . 2008

机译：原生质体融合提高反硝化细菌脱氮效率的实验研究
5. Microscopic studies in nascent frustule formation from protoplasts of the marine pennate apochlorotic diatom Nitzschia alba and production of novel transformation vectors for studies in N. alba and the marine centric photoautotrophic diatom Thalassiosira pseudonana. [D] . Carlson, Jeffrey. 2008

机译：显微研究从海洋半球形脱磷硅藻Nitzschia alba原生质体形成新生的壳层，并生产新的转化载体，用于在N. alba和以海洋为中心的自养硅藻Thalassiosira pseudonana中进行研究。
6. Polyethylene glycol-induced fusion of heat-inactivated and living protoplasts of Bacillus megaterium. [O] . K Fodor, E Demiri, L Alföldi 1978

机译：聚乙二醇诱导的巨大芽孢杆菌热灭活和活原生质体融合。
7. Polyethylene glycol-induced internalization of bacteria into fungal protoplasts: electron microscopic study and optimization of experimental conditions [O] . I Guerra-Tschuschke, I Martín, M T González 1991

机译：聚乙二醇诱导的细菌内化为真菌原生质体：电子显微镜研究和实验条件的优化
8. Electron Microscopic Study of the Morphological Changes in Intestinal Bacteria and Anthracoids Following the Action of Some Phenol Preparations [R] . Skvortsova, E. K., Likhacheva, N. P. 1964

机译：一些苯酚制剂作用后肠道细菌和蒽醌形态变化的电镜观察

Polyethylene glycol-induced internalization of bacteria into fungal protoplasts: electron microscopic study and optimization of experimental conditions.

摘要

著录项

相似文献

相关主题

期刊订阅