Immunological demonstration of a unique 34-dihydroxyphenylacetate 23-dioxygenase in soil Arthrobacter strains.

机译：在土壤节杆菌菌株中独特的34-二羟基苯乙酸23-二加氧酶的免疫学证明。

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Many bacteria biosynthesize 3,4-dihydroxyphenylacetate 2,3-dioxygenases for growth on aromatic acids, but gram-negative organisms have been most extensively studied. A gram-positive strain containing 2,3-dioxygenase activity was identified as Arthrobacter strain Mn-1. The 2,3-dioxygenase from strain Mn-1 was purified to homogeneity by fast protein liquid chromatography with a Mono Q anion-exchange column. Rabbit polyclonal antidioxygenase antibodies were prepared. Ouchterlony double-diffusion and Western blotting (immunoblotting) protocols were used to probe the distribution of the Mn-1 dioxygenase antigen in soil bacteria. Fourteen 2,3-dioxygenase-containing Bacillus and Pseudomonas strains did not contain immunologically cross-reactive proteins. Six of eight Arthrobacter strains contained 2,3-dioxygenase activity, and all of them produced cross-reactive proteins. The data presented here suggest that a unique type of dioxygenase is geographically widespread but is taxonomically confined to Arthrobacter soil bacteria.

机译：许多细菌生物合成3,4-二羟基苯乙酸2,3-二加氧酶以在芳香酸上生长，但是革兰氏阴性生物得到了最广泛的研究。含有2，3-二加氧酶活性的革兰氏阳性菌株被鉴定为节杆菌菌株Mn-1。通过带有Mono Q阴离子交换柱的快速蛋白质液相色谱，将菌株Mn-1中的2,3-二加氧酶纯化至均质。制备了兔多克隆抗双加氧酶抗体。使用双菌落扩散和蛋白质印迹（免疫印迹）方法来检测Mn-1双加氧酶抗原在土壤细菌中的分布。十四个包含2,3-二加氧酶的芽孢杆菌和假单胞菌菌株不包含免疫交叉反应蛋白。八株节杆菌菌株中的六株具有2,3-二加氧酶活性，并且它们全部产生交叉反应蛋白。这里提供的数据表明，独特类型的双加氧酶在地理上广泛分布，但在分类学上仅限于节杆菌土壤细菌。

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期刊名称 Applied and Environmental Microbiology
作者
P E Olson; B Qi; L Que Jr; L P Wackett;
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年(卷),期 1992(58),9
年度 1992
页码 2820–2826
总页数 7
原文格式 PDF
正文语种
中图分类应用微生物学;微生物学;
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专利

1. Immunological demonstration of a unique 3,4-dihydroxyphenylacetate 2,3-dioxygenase in soil Arthrobacter strains. [J] . P E Olson, B Qi, L Que, Applied and Environmental Microbiology . 1992,第9期

机译：在土壤节杆菌菌株中独特的3,4-二羟基苯乙酸2,3-二加氧酶的免疫学证明。
2. Demonstration of shared and unique immunological determinants in enterotoxins from Vibrio cholerae and Escherichia coli. [J] . J D Clements, R A Finkelstein Infection and immunity . 1978,第3期

机译：证明霍乱弧菌和大肠杆菌的肠毒素中共有和独特的免疫学决定因素。
3. Arthrobacter sp. EpRS66 and Arthrobacter sp. EpRS71: Draft Genome Sequences from Two Bacteria Isolated from Echinacea purpurea Rhizospheric Soil [J] . Luana Presta, Marco Fondi, Elena Perrin, Frontiers in Microbiology . 2016,第6期

机译：炭疽菌 sp。 EpRS66和 Arthrobacter sp.。 EpRS71：从紫锥菊根际土壤中分离的两个细菌的基因组序列草案
4. Bioremediation of Atrazine Contaminated Soil by Arthrobacter SP. Strain AD4 [C] . Liu Xia, Li Xihong, Cai Baoli, 2010 4th International Conference on Bioinformatics and Biomedical Engineering . 2010

机译：关节杆菌SP对阿特拉津污染的土壤进行生物修复。应变AD4
5. An investigation into the unique behavior of tropical residual soils. [D] . Zurakowski, Zygi. 2008

机译：研究热带残留土壤的独特行为。
6. 34-Dihydroxyphenylacetate 23-dioxygenase from Klebsiella pneumoniae a Mg(2+)-containing dioxygenase involved in aromatic catabolism. [O] . A Gibello, E Ferrer, M Martín, 1994

机译：来自肺炎克雷伯菌的34-二羟基苯乙酸23-二加氧酶一种参与芳香族分解代谢的含Mg（2+）的二加氧酶。
7. Arthrobacter sp. EpRS66 and Arthrobacter sp. EpRS71: Draft Genome Sequences from Two Bacteria Isolated from Echinacea purpurea Rhizospheric Soil [O] . Luana Presta, Marco Fondi, Elena Perrin, 2016

机译：arthrobacter sp。 EpRs66和arthrobacter sp。 EpRs71：从紫锥菊根际土壤中分离的两种细菌的基因组序列草案
8. Arthrobacter Species as a Prey Cell Reservoir for Nonobligate Bacterial Predators in Soil [R] . Casida, L. E. 1989

机译：节杆菌属物种作为土壤中非垂直细菌捕食者的捕食细胞库

Immunological demonstration of a unique 34-dihydroxyphenylacetate 23-dioxygenase in soil Arthrobacter strains.

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