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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Simultaneous Production and Recovery of Fumaric Acid from Immobilized Rhizopus oryzae with a Rotary Biofilm Contactor and an Adsorption Column
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Simultaneous Production and Recovery of Fumaric Acid from Immobilized Rhizopus oryzae with a Rotary Biofilm Contactor and an Adsorption Column

机译：旋转生物膜接触器和吸附柱从固定根霉米中同时生产和回收富马酸

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An integrated system of simultaneous fermentation-adsorption for the production and recovery of fumaric acid from glucose by Rhizopus oryzae was investigated. The system was constructed such that growing Rhizopus mycelia were self-immobilized on the plastic discs of a rotary biofilm contactor during the nitrogen-rich growth phase. During the nongrowth, production phase, the biofilm was alternately exposed to liquid medium and air upon rotation of the discs in the horizontal fermentation vessel. The product of fermentation, fumaric acid, was removed simultaneously and continuously by a coupled adsorption column, thereby moderating inhibition, enhancing the fermentation rate, and sustaining cell viability. Another beneficial effect of the removal of fumaric acid is release of hydroxyl ions from a polyvinyl pyridine adsorbent into the circulating fermentation broth. This moderates the decrease in pH that would otherwise occur. Polyvinyl pyridine and IRA-900 gave the highest loading for this type of fermentation. This fermentation system is capable of producing fumaric acid with an average yield of 85 g/liter from 100 g of glucose per liter within 20 h under repetitive fed-batch cycles. On a weight yield basis, 91% of the theoretical maximum was obtained with a productivity of 4.25 g/liter/h. This is in contrast to stirred-tank fermentation supplemented with calcium carbonate, whose average weight yield was 65% after 72 h with a productivity of 0.9 g/liter/h. The immobilized reactor was operated repetitively for 2 weeks without loss of biological activity.
机译：研究了一种同时发酵-吸附的集成系统，该系统用于通过米根霉从葡萄糖生产和回收富马酸。构造该系统，以便在富氮生长阶段将生长的根霉菌丝体自固定在旋转生物膜接触器的塑料盘上。在无生长的生产阶段，在水平发酵容器中旋转圆盘时，生物膜交替暴露于液体介质和空气中。发酵产物富马酸通过耦合吸附柱同时连续去除，从而缓和了抑制作用，提高了发酵速率，并维持了细胞活力。去除富马酸的另一个有益效果是将羟基离子从聚乙烯基吡啶吸附剂中释放到循环发酵液中。这可缓和原本会发生的pH降低。聚乙烯基吡啶和IRA-900对此类发酵具有最高的负载量。该发酵系统能够在重复补料分批循环下，在20小时内从每升100克葡萄糖中平均生产85克/升富马酸。以重量计，获得理论最大值的91％，生产率为4.25g /升/ h。这与添加碳酸钙的搅拌罐发酵相反，碳酸钙在72小时后的平均重量产率为65％，生产率为0.9 g / L / h。固定的反应器重复操作2周，而没有丧失生物活性。

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