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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Isolation of Marine Polycyclic Aromatic Hydrocarbon (PAH)-Degrading Cycloclasticus Strains from the Gulf of Mexico and Comparison of Their PAH Degradation Ability with That of Puget Sound Cycloclasticus Strains
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Isolation of Marine Polycyclic Aromatic Hydrocarbon (PAH)-Degrading Cycloclasticus Strains from the Gulf of Mexico and Comparison of Their PAH Degradation Ability with That of Puget Sound Cycloclasticus Strains

机译：来自墨西哥湾的降解海洋多环芳烃（PAH）的环孢菌菌株的分离及其与普吉特海湾环孢菌菌株的降解能力比较

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摘要

Phenanthrene- and naphthalene-degrading bacteria were isolated from four offshore and nearshore locations in the Gulf of Mexico by using a modified most-probable-number technique. The concentrations of these bacteria ranged from 102 to 106 cells per ml of wet surficial sediment in mildly contaminated and noncontaminated sediments. A total of 23 strains of polycyclic aromatic hydrocarbon (PAH)-degrading bacteria were obtained. Based on partial 16S ribosomal DNA sequences and phenotypic characteristics, these 23 strains are members of the genus Cycloclasticus. Three representatives were chosen for a complete phylogenetic analysis, which confirmed the close relationship of these isolates to type strain Cycloclasticus pugetii PS-1, which was isolated from Puget Sound. PAH substrate utilization tests which included high-molecular-weight PAHs revealed that these isolates had similar, broad substrate ranges which included naphthalene, substituted naphthalenes, phenanthrene, biphenyl, anthracene, acenaphthene, and fluorene. Degradation of pyrene and fluoranthene occurred only when the strains were incubated with phenanthrene. Two distinct partial PAH dioxygenase iron sulfur protein (ISP) gene sequences were PCR amplified from Puget Sound and Gulf of Mexico Cycloclasticus strains. Phylogenetic analyses of these sequences revealed that one ISP type is related to the bph type of ISP sequences, while the other ISP type is related to the nah type of ISP sequences. The predicted ISP amino acid sequences for the Gulf of Mexico and Puget Sound strains are identical, which supports the hypothesis that these geographically separated isolates are closely related phylogentically. Cycloclasticus species appear to be numerically important and widespread PAH-degrading bacteria in both Puget Sound and the Gulf of Mexico.
机译：使用改进的最可能数技术从墨西哥湾的四个近海和近海地点分离出降解菲和萘的细菌。在轻度污染和未污染的沉积物中，每毫升湿表层沉积物中这些细菌的浓度范围为10 2 至10 6 细胞。总共获得了23株降解多环芳烃（PAH）的细菌。基于部分16S核糖体DNA序列和表型特征，这23个菌株是Cycloclasticus属的成员。选择了三个代表进行完整的系统发育分析，这证实了这些分离株与从Puget Sound分离的Cycloclasticus pugetii PS-1型菌株的密切关系。包括高分子量PAHs的PAH底物利用率测试表明，这些分离物具有相似，较宽的底物范围，包括萘，取代的萘，菲，联苯，蒽，和芴。 when和荧蒽的降解仅在菌株与菲一起孵育时发生。从普吉特湾和墨西哥湾Cycloclasticus菌株中PCR扩增了两个截然不同的部分PAH双加氧酶铁硫蛋白（ISP）基因序列。这些序列的系统发生分析表明，一种ISP类型与ISP序列的bph类型有关，而另一种ISP类型与ISP序列的nah类型有关。墨西哥湾和普吉特海湾菌株的预测ISP氨基酸序列相同，这支持以下假设：这些地理上分离的分离株在系统发育上密切相关。在普吉特海湾和墨西哥湾，轮回破壁菌菌种在数量上似乎很重要，并且广泛降解PAH。

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