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首页> 外文期刊>International Biodeterioration & Biodegradation >Isolation of a high molecular weight polycyclic aromatic hydrocarbon-degrading strain and its enhancing the removal of HMW-PAHs from heavily contaminated soil
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Isolation of a high molecular weight polycyclic aromatic hydrocarbon-degrading strain and its enhancing the removal of HMW-PAHs from heavily contaminated soil

机译:高分子量多环芳烃降解菌的分离及其促进重污染土壤中HMW-PAHs的去除

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摘要

A high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs)-degrading strain, designed P10, was isolated from PAHs contaminated soil. Strain P10 was preliminarily identified as Kocuria sp. based on the analysis of 16S rRNA gene sequence. Degradation of individual PAHs was investigated in basal salt medium using PAH as sole carbon and energy source. The results showed that strain P10 could degrade several PAHs including HMW-PAHs, such as fluorene, fluoranthene and indeno[1,2,3-cd]pyrene with degrading percentages of 83.2%, 76.5% and 59.6%, respectively, after incubation for 14 days at 25 C. Bioremediation experiments of HMW-PAHs heavily contaminated soil were also carried out using strain P10 as inoculum in weathered plots under natural conditions. The results indicated that strain P10 could significantly enhance the removal of HMW-PAHs from the contaminated soil. The removal percentages were increased by about 2-folds for HMW-PAHs in comparison to the non-inoculated one
机译:从多环芳烃污染土壤中分离出设计为P10的高分子量多环芳烃降解菌株。菌株P10初步鉴定为Kocuria sp。基于对16S rRNA基因序列的分析。使用PAH作为唯一碳和能源,在基础盐培养基中研究了单个PAH的降解。结果显示,菌株P10孵育60℃后,可以降解包括芴,荧蒽和茚并[1,2,3-cd] H等HMW-PAHs的几种PAHs,降解百分率分别为83.2%,76.5%和59.6%。在25°C下放置14天。在自然条件下,还使用P10菌株作为接种物,对HMW-PAHs重度污染的土壤进行了生物修复实验。结果表明,菌株P10可以显着增强HMW-PAHs从污染土壤中的去除。与未接种的HMW-PAH相比,HMW-PAH的去除率提高了约2倍

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