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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Effects of GPD1 Overexpression in Saccharomyces cerevisiae Commercial Wine Yeast Strains Lacking ALD6 Genes
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Effects of GPD1 Overexpression in Saccharomyces cerevisiae Commercial Wine Yeast Strains Lacking ALD6 Genes

机译：GPD1过量表达在酿酒酵母缺乏ALD6基因的商业酵母中的作用。

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摘要

The utilization of Saccharomyces cerevisiae strains overproducing glycerol and with a reduced ethanol yield is a potentially valuable strategy for producing wine with decreased ethanol content. However, glycerol overproduction is accompanied by acetate accumulation. In this study, we evaluated the effects of the overexpression of GPD1, coding for glycerol-3-phosphate dehydrogenase, in three commercial wine yeast strains in which the two copies of ALD6 encoding the NADP+-dependent Mg2+-activated cytosolic acetaldehyde dehydrogenase have been deleted. Under wine fermentation conditions, the engineered industrial strains exhibit fermentation performance and growth properties similar to those of the wild type. Acetate was produced at concentrations similar to that of the wild-type strains, whereas sugar was efficiently diverted to glycerol. The ethanol yield of the GPD1 ald6 industrial strains was 15 to 20% lower than that in the controls. However, these strains accumulated acetoin at considerable levels due to inefficient reduction to 2,3-butanediol. Due to the low taste and odor thresholds of acetoin and its negative sensorial impact on wine, novel engineering strategies will be required for a proper adjustment of the metabolites at the acetaldehyde branch point.
机译：利用酿酒酵母菌株生产过量的甘油并降低乙醇产量是生产乙醇含量降低的葡萄酒的潜在有价值的策略。但是，甘油的过量生产伴随着乙酸盐的积累。在这项研究中，我们评估了编码三个甘油三磷酸脱氢酶的GPD1的过量表达对三种商业葡萄酒酵母菌株的影响，其中两份ALD6拷贝编码NADP + 依赖性Mg 2 + 激活的胞质乙醛脱氢酶已被删除。在葡萄酒发酵条件下，工程化的工业菌株显示出与野生型相似的发酵性能和生长特性。产生的乙酸盐浓度与野生型菌株相似，而糖则有效地转移至甘油。 GPD1 ald6工业菌株的乙醇收率比对照组低15％至20％。然而，由于不能有效地还原成2，3-丁二醇，这些菌株在相当数量的水平上积累了乙醛。由于乙酰丁香的味道和气味阈值低，并且对葡萄酒有负面的感官影响，因此需要新的工程策略来适当调节乙醛分支点的代谢物。

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