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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Yersiniabactin Production by Pseudomonas syringae and Escherichia coli and Description of a Second Yersiniabactin Locus Evolutionary Group
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Yersiniabactin Production by Pseudomonas syringae and Escherichia coli and Description of a Second Yersiniabactin Locus Evolutionary Group

机译：丁香假单胞菌和大肠杆菌生产耶尔西菌素以及第二个耶尔西菌素基因座进化组的描述

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The siderophore and virulence factor yersiniabactin is produced by Pseudomonas syringae. Yersiniabactin was originally detected by high-pressure liquid chromatography (HPLC); commonly used PCR tests proved ineffective. Yersiniabactin production in P. syringae correlated with the possession of irp1 located in a predicted yersiniabactin locus. Three similarly divergent yersiniabactin locus groups were determined: the Yersinia pestis group, the P. syringae group, and the Photorhabdus luminescens group; yersiniabactin locus organization is similar in P. syringae and P. luminescens. In P. syringae pv. tomato DC3000, the locus has a high GC content (63.4% compared with 58.4% for the chromosome and 60.1% and 60.7% for adjacent regions) but it lacks high-pathogenicity-island features, such as the insertion in a tRNA locus, the integrase, and insertion sequence elements. In P. syringae pv. tomato DC3000 and pv. phaseolicola 1448A, the locus lies between homologues of Psyr_2284 and Psyr_2285 of P. syringae pv. syringae B728a, which lacks the locus. Among tested pseudomonads, a PCR test specific to two yersiniabactin locus groups detected a locus in genospecies 3, 7, and 8 of P. syringae, and DNA hybridization within P. syringae also detected a locus in the pathovars phaseolicola and glycinea. The PCR and HPLC methods enabled analysis of nonpathogenic Escherichia coli. HPLC-proven yersiniabactin-producing E. coli lacked modifications found in irp1 and irp2 in the human pathogen CFT073, and it is not clear whether CFT073 produces yersiniabactin. The study provides clues about the evolution and dispersion of yersiniabactin genes. It describes methods to detect and study yersiniabactin producers, even where genes have evolved.
机译：铁载体和毒力耶尔西菌素由丁香假单胞菌产生。耶尔西菌素最初是通过高压液相色谱法（HPLC）检测的。常用的PCR检测证明无效。丁香假单胞菌中耶尔森菌素的产生与位于预测耶尔森菌素基因座中的irp1的存在有关。确定了三个相似发散的耶尔西菌素基因座组：鼠疫耶尔森氏菌组，丁香假单胞菌组和光致发光菌组。丁香假单胞菌和发光假单胞菌的耶尔西菌素基因座组织相似。在丁香假单胞菌pv。番茄DC3000，该基因座具有较高的GC含量（63.4％，而染色体为58.4％，相邻区域为60.1％和60.7％），但是它缺乏高致病性的海岛特征，例如在tRNA基因座中插入整合和插入序列元素。在丁香假单胞菌pv。番茄DC3000和PV。菜豆1448A，位点位于丁香假单胞菌pv的Psyr_2284和Psyr_2285的同源物之间。缺少基因座的丁香针叶B728a。在测试的假单胞菌中，特异于两个耶尔西菌素基因座组的PCR测试检测到丁香假单胞菌的基因种3、7和8，而丁香假单胞菌内的DNA杂交也检测到了小菜蛾和甘氨酸的基因座。 PCR和HPLC方法可以分析非致病性大肠杆菌。经HPLC验证的生产耶尔西菌素的大肠杆菌缺乏在人类病原体CFT073中的irp1和 irp2 中发现的修饰，目前尚不清楚CFT073是否产生耶尔西菌素。该研究为耶尔西菌素基因的进化和分散提供了线索。它描述了检测和研究耶尔西菌素生产者的方法，即使基因已经进化。

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