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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Shear Stress Temperature and Inoculation Concentration Influence the Adhesion of Water-Stressed Helicobacter pylori to Stainless Steel 304 and Polypropylene
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Shear Stress Temperature and Inoculation Concentration Influence the Adhesion of Water-Stressed Helicobacter pylori to Stainless Steel 304 and Polypropylene

机译：剪切应力温度和接种浓度影响水压幽门螺杆菌对304不锈钢和聚丙烯的粘附力

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Although molecular techniques have identified Helicobacter pylori in drinking water-associated biofilms, there is a lack of studies reporting what factors affect the attachment of the bacterium to plumbing materials. Therefore, the adhesion of H. pylori suspended in distilled water to stainless steel 304 (SS304) coupons placed on tissue culture plates subjected to different environmental conditions was monitored. The extent of adhesion was evaluated for different water exposure times, using epifluorescence microscopy to count total cell numbers. High shear stresses—estimated through computational fluid dynamics—negatively influenced the adhesion of H. pylori to the substrata (P < 0.001), a result that was confirmed in similar experiments with polypropylene (P < 0.05). However, the temperature and inoculation concentration appeared to have no effect on adhesion (P > 0.05). After 2 hours, H. pylori cells appeared to be isolated on the surface of SS304 and were able to form small aggregates with longer exposure times. However, the formation of a three-dimensional structure was only very rarely observed. This study suggests that the detection of the pathogen in well water described by other authors can be related to the increased ability of H. pylori to integrate into biofilms under conditions of low shear stress. It will also allow a more rational selection of locations to perform molecular or plate culture analysis for the detection of H. pylori in drinking water-associated biofilms.
机译：尽管分子技术已在与饮用水相关的生物膜中鉴定出幽门螺杆菌，但仍缺乏研究报告哪些因素会影响细菌与管道材料的附着。因此，监测了悬浮在蒸馏水中的幽门螺杆菌对放置在经受不同环境条件的组织培养板上的不锈钢304（SS304）试样的粘附力。使用落射荧光显微镜对总细胞数进行计数，以评估不同暴露时间下的粘附程度。高剪切应力（通过计算流体动力学估算）对幽门螺杆菌对基质的附着力产生了负面影响（P <0.001），这一结果在聚丙烯的类似实验中得到了证实（P <0.05）。然而，温度和接种浓度似乎对附着力没有影响（P> 0.05）。 2小时后，幽门螺杆菌细胞似乎在SS304的表面上分离，并能够形成具有较长暴露时间的小聚集体。但是，很少观察到三维结构的形成。这项研究表明，其他作者描述的在井水中检测病原体可能与幽门螺杆菌在低剪应力条件下整合到生物膜中的能力增强有关。它还将允许对位置进行更合理的选择，以进行分子或平板培养分析，以检测饮用水相关生物膜中的幽门螺杆菌。

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