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Impact of Environmental and Genetic Factors on Biofilm Formation by the Probiotic Strain Lactobacillus rhamnosus GG

机译:环境和遗传因素对益生菌鼠李糖乳杆菌GG生物膜形成的影响

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摘要

Lactobacillus rhamnosus GG (ATCC 53103) is one of the clinically best-studied probiotic organisms. Moreover, L. rhamnosus GG displays very good in vitro adherence to epithelial cells and mucus. Here, we report that L. rhamnosus GG is able to form biofilms on abiotic surfaces, in contrast to other strains of the Lactobacillus casei group tested under the same conditions. Microtiter plate biofilm assays indicated that in vitro biofilm formation by L. rhamnosus GG is strongly modulated by culture medium factors and conditions related to the gastrointestinal environment, including low pH; high osmolarity; and the presence of bile, mucins, and nondigestible polysaccharides. Additionally, phenotypic analysis of mutants affected in exopolysaccharides (wzb), lipoteichoic acid (dltD), and central metabolism (luxS) showed their relative importance in biofilm formation by L. rhamnosus GG.
机译:鼠李糖乳杆菌GG(ATCC 53103)是临床研究最好的益生菌之一。此外,鼠李糖乳杆菌GG在体外对上皮细胞和粘液表现出非常好的粘附性。在这里,我们报告鼠李糖乳杆菌GG能够在非生物表面上形成生物膜,这与在相同条件下测试的干酪乳杆菌组的其他菌株相反。微量滴定板生物膜测定表明,鼠李糖乳杆菌GG的体外生物膜形成受到与胃肠道环境有关的培养基因素和条件(包括低pH)的强烈调节;高渗透压以及胆汁,粘蛋白和不易消化的多糖的存在。此外,对受胞外多糖(wzb),脂磷壁酸(dltD)和中枢代谢(luxS)影响的突变体的表型分析表明,它们在鼠李糖乳杆菌GG生物膜形成中具有相对重要性。

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