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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Detection of Differential Host Susceptibility to the Marine Oomycete Pathogen Eurychasma dicksonii by Real-Time PCR: Not All Algae Are Equal
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Detection of Differential Host Susceptibility to the Marine Oomycete Pathogen Eurychasma dicksonii by Real-Time PCR: Not All Algae Are Equal

机译：实时荧光定量PCR检测海洋卵菌致病菌Eryrychasma dicksonii的宿主敏感性差异：并非所有藻类都是平等的

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In the marine environment, a growing body of evidence points to parasites as key players in the control of population dynamics and overall ecosystem structure. However, their prevalence and impact on marine macroalgal communities remain virtually unknown. Indeed, infectious diseases of seaweeds are largely underdocumented, partly because of the expertise required to diagnose them with a microscope. Over the last few years, however, real-time quantitative PCR (qPCR) has emerged as a rapid and reliable alternative to visual symptom scoring for monitoring pathogens. Thus, we present here a qPCR assay suitable for the detection and quantification of the intracellular oomycete pathogen Eurychasma dicksonii in its ectocarpalean and laminarialean brown algal hosts. qPCR and microscopic observations made of laboratory-controlled cultures revealed that clonal brown algal strains exhibit different levels of resistance against Eurychasma, ranging from high susceptibility to complete absence of symptoms. This observation strongly argues for the existence of a genetic determinism for disease resistance in brown algae, which would have broad implications for the dynamics and genetic structure of natural populations. We also used qPCR for the rapid detection of Eurychasma in filamentous brown algae collected in Northern Europe and South America and found that the assay is specific, robust, and widely applicable to field samples. Hence, this study opens the perspective of combining large-scale disease monitoring in the field with laboratory-controlled experiments on the genome model seaweed Ectocarpus siliculosus to improve our understanding of brown algal diseases.
机译：在海洋环境中，越来越多的证据表明，寄生虫是控制种群动态和总体生态系统结构的关键因素。但是，它们的流行和对海洋大型藻类群落的影响实际上仍然未知。确实，海藻的传染病在很大程度上没有记录，部分是因为用显微镜诊断海藻需要专业知识。然而，在过去的几年中，实时定量PCR（qPCR）已经成为视觉症状评分以监测病原体的一种快速，可靠的替代方法。因此，我们在这里提出了一种qPCR检测方法，适用于检测和定量其外果皮和海带棕色藻类宿主中的卵菌内病原体Drysonchasma dicksonii。 qPCR和实验室控制的培养物的显微镜观察表明，克隆的褐藻菌株对Eurychasma表现出不同程度的抗性，范围从高敏感性到完全没有症状。该观察强烈地证明了褐藻中抗病性的遗传决定论的存在，这将对自然种群的动态和遗传结构产生广泛的影响。我们还使用qPCR快速检测了在北欧和南美收集的丝状褐藻中的真鳞鱼，并发现该方法特异性强，可广泛应用于野外样品。因此，本研究为将大规模的疾病监测与基因组模型海藻Ectocarpus siliculosus的实验室控制实验相结合开辟了前景，以增进我们对褐藻病的认识。

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