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首页> 美国卫生研究院文献>Antimicrobial Agents and Chemotherapy >Decolorization of Malachite Green and Crystal Violet by Waterborne Pathogenic Mycobacteria
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Decolorization of Malachite Green and Crystal Violet by Waterborne Pathogenic Mycobacteria

机译：水性致病性分枝杆菌对孔雀石绿和结晶紫的脱色

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摘要

Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium scrofulaceum, Mycobacterium marinum, and Mycobacterium chelonae tolerate high concentrations of the dyes malachite green and crystal violet. Cells of strains of those species decolorized (reduced) both malachite green and crystal violet. Because decolorized malachite green lacked antimicrobial activity, the resistance of these mycobacteria could be due, in part, to their ability to decolorize the dyes. Small amounts of malachite green and its reduced, decolorized product were detected in the lipid fraction of M. avium strain A5 cells grown in the presence of malachite green, suggesting that a minor component of resistance could be due to sequestering the dyes in the extensive mycobacterial cell surface lipid. The membrane fraction of M. avium strain A5 had at least a fivefold-higher specific decolorization rate than did the crude extract, suggesting that the decolorization activity is membrane associated. The malachite green-decolorizing activity of the membrane fraction of M. avium strain A5 was abolished by either boiling or proteinase exposure, suggesting that the decolorizing activity was due to a protein. Decolorization activity of membrane fractions was stimulated by ferrous ion and inhibited by dinitrophenol and metyrapone.
机译：鸟分枝杆菌，胞内分枝杆菌，鼠分枝杆菌，海藻分枝杆菌和螯合分枝杆菌可耐受高浓度的孔雀石绿和结晶紫染料。这些物种的菌株细胞使孔雀石绿和结晶紫都脱色（还原）。因为脱色的孔雀石绿缺乏抗菌活性，所以这些分枝杆菌的抗性可能部分是由于它们使染料脱色的能力。在孔雀石绿存在下生长的鸟分枝杆菌菌株A5细胞的脂质部分中检测到少量的孔雀石绿及其还原的脱色产物，这表明耐药性的较小部分可能是由于在广泛的分枝杆菌中隔离了染料细胞表面脂质。鸟分枝杆菌菌株A5的膜级分比脱色率至少比粗提物高五倍，这表明脱色活性与膜相关。通过沸腾或蛋白酶暴露消除了鸟分枝杆菌菌株A5的膜部分的孔雀石绿脱色活性，这表明脱色活性是由于蛋白质引起的。亚铁离子刺激膜部分的脱色活性，二硝基苯酚和甲吡酮则抑制了膜部分的脱色活性。

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