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Comparison of Microscopy Culture and Conventional Polymerase Chain Reaction for Detection of Blastocystis sp. in Clinical Stool Samples

机译:显微镜文化和常规聚合酶链反应用于检测Blastocystis sp的比较。在临床凳子样品中

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摘要

We tested 513 stool samples from patients in Sydney, Australia for Blastocystis by using five diagnostic techniques: microscopy of a permanently stained smear using a modified iron-hematoxylin stain, two xenic culture systems (a modified Boeck and Drbohlav's medium and tryptone, yeast extract, glucose, methionine-9 medium), and two published conventional polymerase chain reaction methods specific for the small subunit ribosomal DNA. Ninety-eight (19%) samples were positive for Blastocystis in one or more of the diagnostic techniques. The PCR 2 method was the most sensitive at detecting Blastocystis with a sensitivity of 94%, and the least sensitive was microscopy of the permanent stain (48%). Subtype 3 was the most predominant subtype (present in 43% of samples assigned to this group). This study highlights the low sensitivity of microscopy when used as the sole diagnostic modality for detection of Blastocystis sp.
机译:我们使用五种诊断技术对澳大利亚悉尼患者的513份粪便样本进行了囊胚芽孢杆菌检测:显微镜检查法(使用改良的苏木精染色剂对永久染色的涂片进行显微镜检查),两种氙培养系统(改良的Boeck和Drbohlav培养基和胰蛋白,、酵母提取物,葡萄糖,甲硫氨酸9培养基)和两种已发布的针对小亚基核糖体DNA的常规聚合酶链反应方法。在一种或多种诊断技术中,有98(19%)个样本的囊胚芽孢杆菌呈阳性。 PCR 2方法在检测芽孢杆菌中最敏感,灵敏度为94%,最不敏感的是永久染色的显微镜检查(48%)。亚型3是最主要的亚型(在分配给该组的样本中占43%)。这项研究强调了显微镜作为检测Blastocystis sp的唯一诊断方法时,其灵敏度较低。

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