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首页> 美国卫生研究院文献>American Journal of Translational Research >Adipose-derived mesenchymal stem cells embedded in platelet-rich fibrin scaffolds promote angiogenesis preserve heart function and reduce left ventricular remodeling in rat acute myocardial infarction
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Adipose-derived mesenchymal stem cells embedded in platelet-rich fibrin scaffolds promote angiogenesis preserve heart function and reduce left ventricular remodeling in rat acute myocardial infarction

机译：富含血小板的纤维蛋白支架中嵌入的脂肪间充质干细胞可促进大鼠急性心肌梗死的血管生成保留心脏功能并减少左心室重塑

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Objective: This study tested the hypothesis that autologous adipose-derived mesenchymal stem cells (ADMSCs) embedded in platelet-rich fibrin (PRF) can significant promote myocardial regeneration and repair after acute myocardial infarction (AMI). Summary background: With avoiding the needle-related complications, PRF-embedded autologous ADMSCs graft provides a new effective stem cell-based therapeutic strategy for myocardial repair. Methods: Adult male Sprague-Dawley rats were equally divided (n = 8 per group) into group 1 (sham-operated), group 2 (AMI by ligating left coronary artery), group 3 (AMI+ PRF), and group 4 (AMI+PRF-embedded autologous ADMSCs). RPF with or without ADMSCs was patched on infarct area 1h after AMI induction. All animals were sacrificed on day 42 after echocardiography. Results: Left ventricular (LV) dimension and infarct/fibrotic areas were lowest in group 1, highest in group 2, in group 3 higher than in group 4, whereas LV performance and wall thickness exhibited a reversed pattern in all groups (all p < 0.001). Protein expressions of inflammatory (MMP-9, IL-1β), oxidative, apoptotic (Bax, cleaved PARP), fibrotic (Smad 3, TFG-β), hypertrophic (β-MHC), and heart failure (BNP) biomarkers displayed an identical pattern in infarct/fibrotic areas, whereas the protein expressions of anti-inflammatory (IL-10), anti-apoptotic (Bcl-2), anti-fibrotic (Smad1/5, BMP-2) biomarkers and α-MHC showed an opposite pattern (all p < 0.01). Angiogenic activities (c-Kit+, Sca-1+, CD31+, SDF-1α+, CXCR4+ cells; protein expressions of SDF-1α, CXCR4, VEGF) were highest in group 4 and lowest in group 1 (all p < 0.001). Conclusion: ADMSCs embedded in PRF offered significant benefit in preserving LV function and limiting LV remodeling after AMI.
机译：目的：本研究检验了以下假设：富含血小板纤维蛋白（PRF）的自体脂肪间充质干细胞（ADMSC）可以显着促进急性心肌梗塞（AMI）后的心肌再生和修复。摘要背景：避免针相关并发症，PRF嵌入自体ADMSCs移植提供了一种新的基于干细胞的有效心肌修复治疗策略。方法：将成年雄性Sprague-Dawley大鼠平均分为每组8只，分别为第1组（假手术），第2组（结扎左冠状动脉的AMI），第3组（AMI + PRF）和第4组（AMI） + PRF嵌入的自体ADMSC）。 AMI诱导后1h，有或无ADMSC的RPF均被修补在梗死区域。超声心动图检查后第42天将所有动物处死。结果：组1的左心室（LV）尺寸和梗死/纤维化面积最低，组2最高，组3高于组4，而所有组的左室性能和壁厚均呈现相反的趋势（所有p < 0.001）。炎性（MMP-9，IL-1β），氧化，凋亡（Bax，裂解的PARP），纤维化（Smad 3，TFG-β），肥厚性（β-MHC）和心力衰竭（BNP）生物标志物的蛋白表达均显示梗死/纤维化区域的模式相同，而抗炎（IL-10），抗凋亡（Bcl-2），抗纤维化（Smad1 / 5，BMP-2）生物标志物和α-MHC的蛋白质表达显示相反模式（所有p <0.01）。血管生成活性（c-Kit +，Sca-1 +，CD31 +，SDF-1α+，CXCR4 +细胞；SDF-1α，CXCR4，VEGF的蛋白表达）在第4组中最高，而在第1组中最低（所有p <0.001）。结论：植入PRF中的ADMSCs在维持AMI后左室功能和限制左室重构方面具有显着优势。

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