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首页> 美国卫生研究院文献>Acta Histochemica et Cytochemica >Matrix Metalloproteinase-2 Degrades Fibrillin-1 and Fibrillin-2 of Oxytalan Fibers in the Human Eye and Periodontal Ligaments In Vitro
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Matrix Metalloproteinase-2 Degrades Fibrillin-1 and Fibrillin-2 of Oxytalan Fibers in the Human Eye and Periodontal Ligaments In Vitro

机译：基质金属蛋白酶2降解人眼和牙周膜中的Oxytalan纤维的Fibrillin-1和Fibrillin-2。

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摘要

Oxytalan fibers are distributed in the eye and periodontal ligaments (PDL). The ciliary zonule, known as Zinn’s zonule, in the eye is composed of oxytalan fibers, which are bundles of microfibrils consisting mainly of fibrillin-1 and fibrillin-2. As turnover of oxytalan fibers is slow during life, their degradation mechanism remains unclarified. This study was performed to examine degradation pattern of fibrillin-1 and fibrillin-2 by experimental MMP activation. We cultured human non-pigmented ciliary epithelial cells (HNPCEC) and PDL fibroblasts for 7 days, then treated them with concanavalin A to activate matrix metalloproteinase (MMP)-2, and examined the degradation of fibrillin-1 and fibrillin-2 for 72 hr using immunofluorescence. At 7 days of HNPCEC culture, fibrillin-1-positive fibers were observed, some of which merged with fibrillin-2. After MMP-2 activation, fibrillin-1-positive fibers became thin and disappeared by 72 hr, while fibrillin-2-positive fibers disappeared almost completely within 24 hr. At 7 days of PDL fibroblast culture, fibrillin-1-positive fibers were mostly merged with fibrillin-2. After MMP-2 activation, fibrillin-1-positive fibers became thin by 24 hr and had almost disappeared by 48 hr, while fibrillin-2-positive fibers decreased constantly after 24 hr. A MMP-2 inhibitor completely suppressed these degradations. These results suggest that the patterns of fibrillin-1 and fibrillin-2 degradation differ between the eye and the PDL, possibly reflecting the sensitivity of fibrillin-1 and fibrillin-2 of each type of oxytalan fiber against MMP-2.
机译：Oxytalan纤维分布在眼和牙周膜（PDL）中。眼中的睫状小带由齐纳小带组成，由氧化金属纤维组成，这些金属纤维是一束微纤维，主要由原纤维蛋白-1和原纤维蛋白2组成。由于氧化金属纤维的寿命较慢，因此其降解机理尚不清楚。进行这项研究以检查通过实验性MMP激活的原纤维蛋白1和原纤维蛋白2的降解模式。我们培养了人类无色素的睫状上皮细胞（HNPCEC）和PDL成纤维细胞，共培养7天，然后用伴刀豆球蛋白A处理以激活基质金属蛋白酶（MMP）-2，并检查了72小时的纤维蛋白1和纤维蛋白2的降解使用免疫荧光。 HNPCEC培养7天时，观察到原纤维蛋白1阳性纤维，其中一些与原纤维蛋白2融合。 MMP-2激活后，纤连蛋白1阳性纤维变薄并在72小时内消失，而纤连蛋白2阳性纤维在24小时内几乎完全消失。 PDL成纤维细胞培养7天时，原纤维蛋白1阳性纤维大部分与原纤维蛋白2融合。 MMP-2激活后，原纤维蛋白1阳性纤维在24小时时变细，到48小时时几乎消失，而原纤维蛋白2阳性纤维在24小时后不断减少。 MMP-2抑制剂完全抑制了这些降解。这些结果表明，在眼睛和PDL之间，fibrillin-1和fibrillin-2的降解方式不同，这可能反映了每种类型的oxytalan纤维的fibrillin-1和fibrillin-2对MMP-2的敏感性。

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