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首页> 美国卫生研究院文献>International Journal of Clinical and Experimental Pathology >Effects of simulated microgravity by RCCS on the biological features of Candida albicans
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Effects of simulated microgravity by RCCS on the biological features of Candida albicans

机译：RCCS模拟微重力对白色念珠菌生物学特性的影响

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During the spaceflight, a wide variety of microorganisms may be carried to the outer space by astronauts and aviation component. The yeast Candida albicans is an important opportunistic pathogen responsible for a variety of cutaneous and systemic human infections in human body, and the yeast cell itself could be affected by various stressful environmental factors including the weightless environment. We evaluated the effects of simulated microgravity on biological features of Candida albicans using the rotary cell culture system (RCCS). The growth curves of Candida albicans cultured in RCCS were recorded by spectrophotometer, the morphogenic switches were observed by optical microscope, and the viability of cells exposed to the various concentrations of fluconazole solution was assayed by flow cytometry at 7th, 14th and 21st day of experiment. The results showed that Candida albicans SC5314 under modeled microgravity were manifested as the growth curves leftward-shifted, lag phase shortened, along with logarithmic phase and stationary phase forwarded (P < 0.05). The simulated microgravity increased the growth rate and mycelia formation of Candida albicans. A statistically significant decrease in viability was detected in cells cultured for 7 d, 14 d and 21 d in group of simulated microgravity compared with the control group (P < 0.05). The increase of exposure time to simulate microgravity resulted in the decrease of viability of cells accordingly in same drug concentration compared with the control group. The study demonstrated that the three weeks’ simulated microgravity in RCCS had a noticeable affect on the growth status of mycelia and spores and the morphogenic switches of Candida albicans, meanwhile, the yeast cells under simulated microgravity showed an increased antifungal susceptibility to fluconazole.
机译：在太空飞行期间，宇航员和航空人员会将各种各样的微生物带到外层空间。酵母白色念珠菌是重要的机会病原体，负责人体中各种皮肤和全身性人类感染，并且酵母细胞本身可能受到各种压力环境因素（包括失重环境）的影响。我们使用旋转细胞培养系统（RCCS）评估了模拟微重力对白色念珠菌生物学特性的影响。用分光光度计记录在RCCS中培养的白色念珠菌的生长曲线，通过光学显微镜观察形态发生开关，并在实验的第7、14和21天通过流式细胞术测定暴露于不同浓度氟康唑溶液的细胞的活力。 。结果表明，在微重力作用下，白色念珠菌SC5314的生长曲线向左移动，滞后期缩短，对数期和固定期均向前推进（P <0.05）。模拟的微重力提高了白色念珠菌的生长速率和菌丝体形成。与对照组相比，模拟微重力组培养7 d，14 d和21 d的细胞的活力降低了，具有统计学意义（P <0.05）。与对照组相比，在相同药物浓度下，模拟微重力的暴露时间增加导致细胞活力相应降低。研究表明，在RCCS中进行的三周模拟微重力处理对菌丝体和孢子的生长状况以及白色念珠菌的形态发生变化有显着影响，同时，在模拟微重力处理下的酵母细胞对氟康唑的抗真菌敏感性增加。

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