• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Overexpression of a Wheat Aquaporin Gene TdPIP2;1 Enhances Salt and Drought Tolerance in Transgenic Durum Wheat cv. Maali
【2h】

Overexpression of a Wheat Aquaporin Gene TdPIP2;1 Enhances Salt and Drought Tolerance in Transgenic Durum Wheat cv. Maali

机译：小麦水通道蛋白基因TdPIP2; 1的过表达增强了转基因硬质小麦cv的耐盐和耐旱性。马阿里

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, we generated transgenic durum wheat cv. Maali overexpressing the wheat plasma membrane aquaporin TdPIP2;1 gene under the control of PrTdPIP2;1 promoter or under the constitutive PrCaMV35S promoter. Histochemical analysis of the fusion PrTdPIP2;1::TdPIP2;1::GusA in wheat plants showed that the β-glucuronidase (GUS) activity was detected in the leaves, stems and roots of stably transformed wheat T3 plants. Our results showed that transgenic wheat lines overexpressing the TdPIP2;1 gene exhibited improved germination rates and biomass production and retained low Na+ and high K+ concentrations in their shoots under high salt and osmotic stress conditions. In a long-term study under greenhouse conditions on salt or drought stress, transgenic TdPIP2;1 lines produced filled grains, whereas wild-type (WT) plants either died at the vegetative stage under salt stress or showed drastically reduced grain filling under drought stress. Performing real time RT-PCR experiments on wheat plants transformed with the fusion PrTdPIP2;1::GusA, we showed an increase in the accumulation of GusA transcripts in the roots of plants challenged with salt and drought stress. Study of the antioxidant defence system in transgenic wheat TdPIP2;1 lines showed that these lines induced the antioxidative enzymes Catalase (CAT) and Superoxide dismutase (SOD) activities more efficiently than the WT plants, which is associated with lower malondialdehyde and hydrogen peroxide contents. Taken together, these results indicate the high potential of the TdPIP2;1 gene for reducing water evaporation from leaves (water loss) in response to water deficit through the lowering of transpiration per unit leaf area (stomatal conductance) and engineering effective drought and salt tolerance in transgenic TdPIP2;1 lines.
机译：在这项研究中，我们生成了转基因硬质小麦cv。在PrTdPIP2; 1启动子或组成型PrCaMV35S启动子的控制下，Maali过表达小麦质膜水通道蛋白TdPIP2; 1基因。小麦植物中融合的PrTdPIP2; 1 :: TdPIP2; 1 :: GusA的组织化学分析表明，在稳定转化的小麦T3植物的叶，茎和根中检测到β-葡萄糖醛酸酶（GUS）活性。结果表明，在高盐条件下，过表达TdPIP2; 1基因的转基因小麦品系发芽率和生物量提高，且Na + 和K + 浓度低。和渗透压力条件。在温室条件下对盐或干旱胁迫的长期研究中，转基因TdPIP2; 1品系产生了饱满的谷粒，而野生型（WT）植物要么在盐胁迫下在营养阶段死亡，要么在干旱胁迫下显示出大幅减少的籽粒填充。在用融合的PrTdPIP2; 1 :: GusA转化的小麦植株上进行实时RT-PCR实验，我们发现在受盐和干旱胁迫的植株根中，GusA转录本的积累量增加了。对转基因小麦TdPIP2; 1系的抗氧化防御系统的研究表明，这些系比野生型植物更有效地诱导了抗氧化酶过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性，这与较低的丙二醛和过氧化氢含量有关。综上所述，这些结果表明，TdPIP2; 1基因具有很高的潜力，可通过降低单位叶面积的蒸腾量（气孔导度）和工程有效的干旱和耐盐性来减少水分亏缺，从而减少叶片水分蒸发（失水）在转基因TdPIP2; 1系中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号