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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Expression Profile of Genes Regulating Steroid Biosynthesis and Metabolism in Human Ovarian Granulosa Cells—A Primary Culture Approach
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Expression Profile of Genes Regulating Steroid Biosynthesis and Metabolism in Human Ovarian Granulosa Cells—A Primary Culture Approach

机译：调节人类卵巢颗粒细胞中类固醇生物合成和代谢的基因的表达谱—初步培养方法

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Because of the deep involvement of granulosa cells in the processes surrounding the cycles of menstruation and reproduction, there is a great need for a deeper understanding of the ways in which they function during the various stages of those cycles. One of the main ways in which the granulosa cells influence the numerous sex associated processes is hormonal interaction. Expression of steroid sex hormones influences a range of both primary and secondary sexual characteristics, as well as regulate the processes of oogenesis, folliculogenesis, ovulation, and pregnancy. Understanding of the exact molecular mechanisms underlying those processes could not only provide us with deep insight into the regulation of the reproductive cycle, but also create new clinical advantages in detection and treatment of various diseases associated with sex hormone abnormalities. We have used the microarray approach validated by RT-qPCR, to analyze the patterns of gene expression in primary cultures of human granulosa cells at days 1, 7, 15, and 30 of said cultures. We have especially focused on genes belonging to ontology groups associated with steroid biosynthesis and metabolism, namely “Regulation of steroid biosynthesis process” and “Regulation of steroid metabolic process”. Eleven genes have been chosen, as they exhibited major change under a culture condition. Out of those, ten genes, namely STAR, SCAP, POR, SREBF1, GFI1, SEC14L2, STARD4, INSIG1, DHCR7, and IL1B, belong to both groups. Patterns of expression of those genes were analyzed, along with brief description of their functions. That analysis helped us achieve a better understanding of the exact molecular processes underlying steroid biosynthesis and metabolism in human granulosa cells.
机译：由于颗粒细胞深入参与月经和生殖周期的过程，因此迫切需要更深入地了解它们在月经周期各个阶段的功能方式。颗粒细胞影响众多性相关过程的主要方式之一是激素相互作用。类固醇性激素的表达影响一系列主要和次要性特征，并调节卵子发生，卵泡形成，排卵和怀孕的过程。了解这些过程背后的确切分子机制不仅可以使我们对生殖周期的调控有更深入的了解，而且可以在检测和治疗与性激素异常有关的各种疾病中创造新的临床优势。我们已经使用了通过RT-qPCR验证的微阵列方法，以分析在所述培养的第1、7、15和30天人颗粒细胞的原代培养中的基因表达模式。我们特别关注与类固醇生物合成和代谢相关的属于本体组的基因，即“类固醇生物合成过程的调控”和“类固醇代谢过程的调控”。选择了11个基因，因为它们在培养条件下表现出重大变化。在这十个基因中，STAR，SCAP，POR，SREBF1，GFI1，SEC14L2，STARD4，INSIG1，DHCR7和IL1B属于这两个基因组。分析了这些基因的表达模式，并简要描述了它们的功能。该分析帮助我们更好地了解了人类颗粒细胞中类固醇生物合成和代谢的确切分子过程。

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