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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Study on the Correlation between Gene Expression and Enzyme Activity of Seven Key Enzymes and Ginsenoside Content in Ginseng in Over Time in Ji’an China
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Study on the Correlation between Gene Expression and Enzyme Activity of Seven Key Enzymes and Ginsenoside Content in Ginseng in Over Time in Ji’an China

机译：吉安市人参中7种关键酶的基因表达与酶活性及人参皂苷含量的相关性研究

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摘要

Panax ginseng is a traditional medicine. Fresh ginseng is one of the most important industries related to ginseng development, and fresh ginseng of varying ages has different medicinal properties. Previous research has not systematically reported the correlation between changes in key enzyme activity with changes in ginsenoside content in fresh ginseng over time. In this study, for the first time, we use ginseng samples of varying ages in Ji’an and systematically reported the changes in the activity of seven key enzymes (HMGR, FPS, SS, SE, DS, CYP450, and GT). We investigated the content of ginsenoside and gene expression of these key enzymes. Ginsenoside content was measured using HPLC. HPLC, GC-MS, and LC-MS were combined to measure the enzyme activity of the key enzymes. Quantitative PCR was used in the investigation of gene expression. By analyzing the correlation between the enzyme activity and the transcription level of the key enzymes with ginsenoside content, we found that DS and GT enzyme activities are significantly correlated with the ginsenoside content in different ages of ginseng. Our findings might provide a new strategy to discriminate between ginseng of different years. Meanwhile, this research provides important information for the in-depth study of ginsenoside biosynthesis.
机译：人参是传统药物。鲜人参是与人参发展相关的最重要的产业之一，不同年龄的鲜人参具有不同的药用特性。先前的研究尚未系统地报道关键酶活性的变化与新鲜人参中人参皂苷含量随时间的变化之间的相关性。在这项研究中，我们首次使用了吉安市不同年龄的人参样品，系统地报告了7种关键酶（HMGR，FPS，SS，SE，DS，CYP450和GT）的活性变化。我们调查了人参皂甙的含量和这些关键酶的基因表达。人参皂苷含量使用HPLC测量。结合HPLC，GC-MS和LC-MS来测量关键酶的酶活性。定量PCR用于研究基因表达。通过分析人参皂苷含量的关键酶的酶活性与转录水平之间的相关性，我们发现DS和GT酶活性与人参年龄不同时的人参皂苷含量显着相关。我们的发现可能提供一种区分不同年份人参的新策略。同时，该研究为人参皂苷生物合成的深入研究提供了重要的信息。

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