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首页> 美国卫生研究院文献>Journal of Nematology >Low-Temperature Scanning Electron Microscope Observations of theMeloidogyne incognita Egg Mass: The Gelatinous Matrix andEmbryo Development
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Low-Temperature Scanning Electron Microscope Observations of theMeloidogyne incognita Egg Mass: The Gelatinous Matrix andEmbryo Development

机译：低温扫描电子显微镜观察根结线虫卵团：胶状基质和胚胎发育

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摘要

The root-knot nematode Meloidogyne incognita was cultured monoxenically on excised tomato roots. Galls and egg masses were observed daily using a light microscope. Two phases were distinguished in the gelatinous matrix of the egg mass: a translucent, amorphous material on the surface of the egg mass and a denser, layered phase in which nematode eggs were deposited. Egg masses were also cryofixed, fractured, and observed as frozen, hydrated specimens on a cold stage in a scanning electron microscope (SEM). In the SEM, the layered phase appeared as a meshwork of fibrils that became more loosely associated as the gelatinous matrix aged: Small pearl-like bodies were observed along the fibers of gelatinous matrix. The egg shell surface and several stages of embryo development, including the one-cell stage, initial cleavages, blastula, gastrula, tadpole stage, elongation, and molt of the first-stage juvenile within the egg shell, were observed and photographed with this technique. The developmental events observed were consistent with those described in other nematode species with different techniques.
机译：将根结线虫Meloidogyne incognita在切下的番茄根上进行异种培养。每天使用光学显微镜观察胆囊和卵团。卵团的凝胶状基质中有两个相：卵团表面上的半透明无定形物质和线虫卵沉积的较密的分层相。鸡蛋块也被冷冻固定，破碎，并在寒冷的扫描电子显微镜（SEM）上观察为冷冻，水合的标本。在SEM中，层状相表现为原纤维的网状结构，随着凝胶状基质的老化而变得松散地结合在一起：沿着凝胶状基质的纤维观察到小的珍珠状体。观察并拍摄了卵壳表面和胚胎发育的几个阶段，包括卵壳中第一阶段幼体的单细胞阶段，初始卵裂，囊胚，胃小肠，t阶段，伸长和蜕皮。 。观察到的发育事件与使用不同技术的其他线虫物种中描述的事件一致。

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