• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Cells Tissues Organs >Interwoven Four-Compartment Capillary Membrane Technology for Three-Dimensional Perfusion with Decentralized Mass Exchange to Scale Up Embryonic Stem Cell Culture
【2h】

Interwoven Four-Compartment Capillary Membrane Technology for Three-Dimensional Perfusion with Decentralized Mass Exchange to Scale Up Embryonic Stem Cell Culture

机译：交织四室毛细管膜技术的三维灌注与分散质量交换以扩大胚胎干细胞培养。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We describe hollow fiber-based three-dimensional (3D) dynamic perfusion bioreactor technology for embryonic stem cells (ESC) which is scalable for laboratory and potentially clinical translation applications. We added 2 more compartments to the typical 2-compartment devices, namely an additional media capillary compartment for countercurrent ‘arteriovenous’ flow and an oxygenation capillary compartment. Each capillary membrane compartment can be perfused independently. Interweaving the 3 capillary systems to form repetitive units allows bioreactor scalability by multiplying the capillary units and provides decentralized media perfusion while enhancing mass exchange and reducing gradient distances from decimeters to more physiologic lengths of <1 mm. The exterior of the resulting membrane network, the cell compartment, is used as a physically active scaffold for cell aggregation; adjusting intercapillary distances enables control of the size of cell aggregates. To demonstrate the technology, mouse ESC (mESC) were cultured in 8- or 800-ml cell compartment bioreactors. We were able to confirm the hypothesis that this bioreactor enables mESC expansion qualitatively comparable to that obtained with Petri dishes, but on a larger scale. To test this, we compared the growth of 129/SVEV mESC in static two-dimensional Petri dishes with that in 3D perfusion bioreactors. We then tested the feasibility of scaling up the culture. In an 800-ml prototype, we cultured approximately 5 × 109 cells, replacing up to 800 conventional 100-mm Petri dishes. Teratoma formation studies in mice confirmed protein expression and gene expression results with regard to maintaining ‘stemness’ markers during cell expansion.
机译：我们描述了基于空心纤维的三维（3D）动态灌注生物反应器技术的胚胎干细胞（ESC），可扩展为实验室和潜在的临床翻译应用程序。我们在典型的2隔室设备中增加了2个隔室，即用于逆流“动静脉”流动的附加介质毛细管隔室和充氧毛细管室。每个毛细血管室可以独立灌注。通过将3个毛细管系统交织在一起形成重复单元，可以通过增加毛细管单元来实现生物反应器的可扩展性，并提供分散的介质灌注，同时增强质量交换并减少从分米到小于1 mm的更大生理长度的梯度距离。所得膜网络的外部，即细胞室，被用作细胞聚集的物理活性支架。调节毛细管间距离可以控制细胞聚集体的大小。为了证明该技术，将小鼠ESC（mESC）培养在8或800 ml细胞室生物反应器中。我们能够证实这一假说，即该生物反应器能够使mESC扩增在质量上与使用培养皿获得的mESC相当，但规模更大。为了测试这一点，我们比较了静态二维培养皿中129 / SVEV mESC和3D灌注生物反应器中129 / SVEV mESC的生长。然后，我们测试了扩大文化规模的可行性。在一个800毫升的原型中，我们培养了大约5×10 9 细胞，替代了多达800个常规的100毫米培养皿。小鼠畸胎瘤形成研究证实了在细胞扩增过程中维持“干”标记的蛋白质表达和基因表达结果。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号