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首页> 美国卫生研究院文献>Experimental and Therapeutic Medicine >Epidermal growth factor treatment has protective effects on the integrity of the blood-brain barrier against cerebral ischemia injury in bEnd3 cells
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Epidermal growth factor treatment has protective effects on the integrity of the blood-brain barrier against cerebral ischemia injury in bEnd3 cells

机译：表皮生长因子治疗对bEnd3细胞中抗脑缺血损伤的血脑屏障的完整性具有保护作用

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Tight junctions (TJs) serve an important role in maintaining the integrity of the blood-brain barrier (BBB), while neurological disorders, including ischemic stroke, induce TJ disruption and increase BBB permeability; results include edema formation and hemorrhage transformation. Cerebral endothelium protection presents a promising approach in ischemic stroke therapy. In the current study, protective effects of the epidermal growth factor (EGF) on ischemia-induced disruption of BBB integrity were examined using an oxygen-glucose deprivation (OGD) model in bEnd3 cells. Expression levels of claudin-5 and TJ protein-1 (ZO-1) were determined by reverse transcription-quantitative polymerase chain reaction and western blot analysis. Cell viability was evaluated by cell counting kit-8 assay and the endothelial permeability of Lucifer yellow (LY) was assessed using Transwell assays. The results revealed that post-ischemia administration of EGF (250 ng/ml) significantly attenuated the decrease in mRNA (P<0.05) and protein (P<0.01) expression levels of claudin-5 and ZO-1, and the increase in endothelial permeability of LY (P<0.05) induced by 4 h OGD exposure followed by 24 h reoxygenation. In addition, EGF did not significant affect cell viability. The current study suggested a potential of EGF to improve BBB integrity against ischemic injury by upregulating the expression of TJ proteins and reducing endothelial permeability.
机译：紧密连接（TJ）在维持血脑屏障（BBB）完整性方面起着重要作用，而包括缺血性中风在内的神经系统疾病会诱导TJ破坏并增加BBB通透性；结果包括水肿形成和出血转化。脑内皮保护是缺血性中风治疗中一种有前途的方法。在当前研究中，使用bEnd3细胞中的氧-葡萄糖剥夺（OGD）模型检查了表皮生长因子（EGF）对缺血诱导的BBB完整性破坏的保护作用。通过反转录-定量聚合酶链反应和蛋白质印迹分析确定claudin-5和TJ蛋白-1（ZO-1）的表达水平。通过细胞计数试剂盒8测定法评估细胞生存力，并使用Transwell测定法评估路西法黄（LYifer）的内皮通透性。结果表明，缺血后给予EGF（250 ng / ml）可以显着抑制claudin-5和ZO-1的mRNA表达（P <0.05）和蛋白质（P <0.01）的表达降低以及内皮细胞的升高OGD暴露4 h后再充氧24 h诱导的LY的通透性（P <0.05）。此外，EGF对细胞活力没有显着影响。当前的研究表明，EGF具有通过上调TJ蛋白表达和降低内皮通透性来改善BBB抵抗缺血性损伤的完整性的潜力。

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