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Effect of vitamin D on the learning and memory ability of FGR rat and NMDA receptor expression in hippocampus

机译：维生素D对FGR大鼠学习记忆能力和海马NMDA受体表达的影响。

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The aim of this study is to investigate the effect of vitamin D (VD) on the learning and memory ability of fetal growth restriction (FGR) rat and the expression of NMDA receptor in hippocampus. The FGR models of rat were established through passive smoking, and divided into two groups randomly, i.e. the control group and the observation group. Rats were fed regular diet with the addition of VD in the observation group. The serum level of 25(OH)-D3 was assayed in both groups of the rats at different time points using ELISA, and the learning and memory ability of rat aged 30 days were evaluated using Morris water maze and passive avoidance test. In addition, we also compared the brain weight of rats at different age and detected the mRNA expression of NRI subunit of NMDA receptor of hippocampus of rats aged 30 days using quantitative RT-PCR. After 7 days, the serum level of 25(OH)D3 in rats of the observation group was significantly higher than that of the control group (P<0.05); during the Morris water maze, we found no significant difference in comparison of the latency between the two groups in the first 2 days (P>0.05), but from the 3rd day, the latency of the observation group was shorter than that in the control group (P<0.05); in the passive avoidance test, no significant difference was identified when comparing the electric shock times between the two groups in the first 2 days, but from the 3rd day, the electric shock times in the observation group were significantly lower than those in the control group (P<0.05); the brain weight of rats in the observation group on the 1st, 7th and 14th day were all lower than those in the control group (P<0.05), but the comparison of brain weight at 21st and 30th day showed no significant difference (P>0.05) between the two groups. We also found that the mRNA and protein expression of NRI subunit of NMDA receptor in hippocampus was significantly higher in the observation group than in the control group (P<0.05). VD can increase the learning and memory ability of FGR rats, significantly ameliorating the cognitive dysfunction of FGR rat and improving the learning and memory ability of rats, which may be related to the upregulation of NRI subunit of NMDA receptor.
机译：本研究的目的是研究维生素D（VD）对胎儿生长受限（FGR）大鼠的学习和记忆能力以及海马NMDA受体表达的影响。通过被动吸烟建立大鼠的FGR模型，随机分为两组，即对照组和观察组。在观察组中，给大鼠常规饮食加VD。采用酶联免疫吸附测定法（ELISA）检测两组大鼠在不同时间点的血清25（OH）-D3水平，采用莫里斯水迷宫法和被动回避测试法评价30日龄大鼠的学习记忆能力。此外，我们还比较了不同年龄大鼠的脑重量，并使用定量RT-PCR检测了30天龄大鼠海马NMDA受体NRI亚基的mRNA表达。 7天后，观察组大鼠血清25（OH）D3水平明显高于对照组（P <0.05）。在莫里斯水迷宫中，我们发现前两天两组的潜伏期比较没有显着差异（P> 0.05），但是从第三天开始，观察组的潜伏期比对照组短。组（P <0.05）；在被动回避测试中，比较前两天两组的电击时间没有发现显着差异，但是从第三天起，观察组的电击时间明显低于对照组。 （P <0.05）；观察组大鼠在第1、7、14天的脑质量均低于对照组（P <0.05），但在第21、30天的脑质量比较无明显差异（P> 0.05）。两组之间的差异为0.05）。我们还发现，观察组海马NMDA受体NRI亚单位的mRNA和蛋白表达明显高于对照组（P <0.05）。 VD可以增加FGR大鼠的学习记忆能力，明显改善FGR大鼠的认知功能障碍，改善大鼠的学习记忆能力，这可能与NMDA受体NRI亚单位的上调有关。

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