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首页> 美国卫生研究院文献>Experimental and Therapeutic Medicine >Human adipose-derived stem cell adipogenesis induces paracrine regulation of the invasive ability of MCF-7 human breast cancer cells in vitro
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Human adipose-derived stem cell adipogenesis induces paracrine regulation of the invasive ability of MCF-7 human breast cancer cells in vitro

机译：人脂肪来源的干细胞成脂诱导体外分泌调节MCF-7人乳腺癌细胞的侵袭能力

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The aim of this study was to determine the effects of paracrine regulation on the invasive ability of MCF-7 human breast cancer cells through human adipose-derived stem cell (hADSC) adipogenesis. hADSC differentiation of the third and fourth passages of cells was induced in different induction media: osteogenic, adipogenic and chondrogenic. Transwell migration assays in the differently conditioned media, flow cytometry, enzyme-linked immunosorbent assay and western blot analysis for selected cytokines were performed. The flow cytometric analysis demonstrated positive expression of CD29, CD44 and CD105, while expression of CD34 and CD45 was not identified. The transwell migration assay showed that the invasive ability of MCF-7 cells was significantly enhanced during hADSC adipogenesis. hADSCs exerted a significantly positive effect on the invasive activity of MCF-7 cells during adipo-genesis. The results indicate that the high expression levels of activating protein 2 (aP2) in MCF-7 and adipocytes induced for 12 days may be associated with cell growth, invasion and metastasis. Peroxisome proliferator-activated receptor γ may be involved in fatty syntheses during adipogenic initiation and following adipogenic differentiation, possibly acting as a protection factor resulting in cell maturation and differentiation. This study also demonstrated that the expression of vascular endothelial growth factor was repressed by hADSCs, while that of matrix metalloproteinase-2 and urokinase-type plasminogen activator was increased to a significant level.
机译：这项研究的目的是确定旁分泌调节通过人脂肪来源的干细胞（hADSC）成脂作用对MCF-7人乳腺癌细胞侵袭能力的影响。在不同的诱导培养基中诱导了第三和第四代细胞的hADSC分化：成骨，成脂和成软骨。在不同条件的培养基中进行Transwell迁移测定，流式细胞仪，酶联免疫吸附测定和蛋白质印迹分析，以选择细胞因子。流式细胞仪分析显示CD29，CD44和CD105阳性表达，而未鉴定CD34和CD45的表达。 Transwell迁移分析表明，在hADSC成脂过程中，MCF-7细胞的侵袭能力显着增强。在脂肪生成过程中，hADSCs对MCF-7细胞的侵袭活性发挥了显着的积极作用。结果表明，在诱导的12天中，MCF-7和脂肪细胞中活化蛋白2（aP2）的高表达水平可能与细胞生长，侵袭和转移有关。过氧化物酶体增殖物激活的受体γ可能在脂肪形成过程中和脂肪形成分化后参与脂肪合成，可能是导致细胞成熟和分化的保护因子。这项研究还表明，hADSCs抑制了血管内皮生长因子的表达，而基质金属蛋白酶2和尿激酶型纤溶酶原激活剂的表达则显着增加。

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