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首页> 美国卫生研究院文献>Journal of Chromatographic Science >Isolation of Flavonoids From Wild Aquilaria sinensis Leavesby an Improved Preparative High-Speed Counter-Current ChromatographyApparatus
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Isolation of Flavonoids From Wild Aquilaria sinensis Leavesby an Improved Preparative High-Speed Counter-Current ChromatographyApparatus

机译：野生沉香叶中黄酮类化合物的分离改进的制备型高速逆流色谱仪器

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摘要

Four flavonoids including apigenin-7,4′-dimethylether, genkwanin, quercetin, and kaempferol were isolated in a preparative or semi-preparative scale from the leaves of wild Aquilaria sinensis using an improved preparative high-speed counter-current chromatography apparatus. The separations were performed with a two-phase solvent system composed of hexane–ethyl acetate, methanol–water at suitable volume ratios. The obtained fractions were analyzed by HPLC, and the identification of each target compound was carried out by ESI-MS and NMR. The yields of the above four target flavonoids were 4.7, 10.0, 11.0 and 4.4%, respectively. All these four flavonoids exhibited nitrite scavenging activities with the clearance rate of 12.40 ± 0.20%, 5.84 ± 0.03%, 28.10 ± 0.17% and 5.19 ± 0.11%, respectively. Quercetin was originally isolated from the Thymelaeaceae family, while kaempferol was isolated from the Aquilaria genus for the first time. In cytotoxicity test these two flavonoids exhibited moderate inhibitory activities against HepG2 cells with the IC50 values of 12.54 ± 1.37 and 38.63 ± 4.05 μM, respectively.
机译：使用改进的制备型高速逆流色谱仪从中华沉香的叶子中以制备或半制备规模分离了包括芹菜素-7,4'-二甲醚，genkwanin，槲皮素和山奈酚的四种类黄酮。分离使用两相溶剂系统进行，该系统由适当体积比的己烷-乙酸乙酯，甲醇-水组成。通过HPLC分析获得的级分，并通过ESI-MS和NMR进行每种目标化合物的鉴定。以上四种目标类黄酮的产率分别为4.7％，10.0％，11.0％和4.4％。这四种类黄酮均具有清除亚硝酸盐的活性，清除率分别为12.40±0.20％，5.84±0.03％，28.10±0.17％和5.19±0.11％。槲皮素最初是从百里香科中分离出来的，而山奈酚则是第一次从沉香属中分离出来的。在细胞毒性测试中，这两种类黄酮对HepG2细胞表现出中等抑制活性，IC50值分别为12.54±1.37和38.63±4.05μM。

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