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首页> 美国卫生研究院文献>Journal of Medical Entomology >Organ-Associated Muscles in Aedes albopictus (Diptera: Culicidae) Respond Differentially to Sindbis Virus
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Organ-Associated Muscles in Aedes albopictus (Diptera: Culicidae) Respond Differentially to Sindbis Virus

机译：白纹伊蚊（双翅目：Cu科）的器官相关的肌肉对信德比斯病毒的反应不同。

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Differential host cell responses to the alphavirus Sindbis were observed in visceral muscles of the adult female mosquito Aedes albopictus. Following intrathoracic inoculation with SIN, muscles associated with the midgut, hindgut, and ovary resulted in clearance, persistence, and refractoriness to virus, respectively. Prominent sarcomeres characteristic of myofilaments were identified in muscles associated with these three organs by phalloidin labeling of actin, confirming these cells as muscle. The location of virus antigen mimicked the distribution of actin in both mid- and hindgut-associated muscles. Furthermore, these myofilaments remained intact following virus clearance from midgut muscles and during virus persistence in hindgut muscles. Changes in the temporal onset of virus antigen following high titer inoculum compared with standard titer inoculum was observed in anterior midgut muscles, but not in muscles associated with the posterior midgut or hindgut. Muscle bundles closely approximated the gut surface, while a wispy association was displayed at the ovary surface. Prominent ultrastructural differences were observed in the basal lamina attached to the gut compared with the ovary. Additionally, ultrastructural evidence for virus-associated pathology was observed in gut-associated muscles and gut epithelium. Visceral muscles, all composed of the same tissue type, but associated to three different organs in the insect abdomen, responded differentially to Sindbis. We speculate that variations in structure, function or physiology and ultrastructure inherent to insect host cells or organs interactions reflect the complicated milieu of the organism and contribute to differential virus phenotypic expression in muscle cells.
机译：在成年雌性蚊白纹伊蚊的内脏肌肉中观察到了对α病毒Sindbis的不同宿主细胞反应。胸腔内接种SIN后，与中肠，后肠和卵巢相关的肌肉分别导致清除，持久性和对病毒的抵抗性。通过用鬼笔环肽标记肌动蛋白，在与这三个器官相关的肌肉中发现了肌丝的突出性肉瘤特征，确认这些细胞为肌肉。病毒抗原的位置模仿了肌动蛋白在中肠和后肠相关肌肉中的分布。此外，在中肠肌肉清除病毒后以及后肠肌肉中的病毒持续存在期间，这些肌丝仍保持完整。与标准滴度接种物相比，高滴度接种物后病毒抗原在时间上的变化在前中肠肌肉中观察到，但在后中肠或后肠相关的肌肉中没有观察到。肌肉束非常接近肠表面，而在卵巢表面则显示出稀疏的联想。与卵巢相比，在附着于肠的基底薄层中观察到明显的超微结构差异。此外，在肠道相关的肌肉和肠道上皮中观察到了病毒相关病理的超微结构证据。内脏肌肉全部由相同的组织类型组成，但与昆虫腹部的三个不同器官相关，对辛德比斯的反应不同。我们推测昆虫宿主细胞或器官相互作用固有的结构，功能或生理学和超微结构的变化反映了生物体的复杂环境，并促进了肌肉细胞中病毒表型的差异表达。

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