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首页> 美国卫生研究院文献>Journal of Experimental Botany >Transcriptomic analysis of grain filling in rice inferior grains under moderate soil drying
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Transcriptomic analysis of grain filling in rice inferior grains under moderate soil drying

机译：土壤适度干燥下劣质水稻籽粒灌浆的转录组学分析。

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摘要

Moderate soil drying imposed at the post-anthesis stage significantly increases starch accumulation in inferior grains of rice, but how this process is regulated at the level of gene expression remains unclear. In this study, we applied moderate drying (MD) treatments to the soil at the post-anthesis stage and followed the dynamics of the conversion process of soluble sugars to starch in inferior grains using RNA-seq analysis. An elevated level of ABA induced by MD was consistently associated with down-regulation of ABA8ox2, suggesting that lower expression of this gene may be responsible for the higher ABA content, potentially resulting in better filling in inferior grains. In addition, MD treatments up-regulated genes encoding five key enzymes involved sucrose-to-starch conversion and increased the activities of enzymes responsible for soluble-sugar reduction and starch accumulation in inferior grains. Differentially expressed transcription factors, including NAC, GATA, WRKY, and M-type MADS, were predicted to interact with other proteins in mediating filling of inferior grains as a response to MD. Transient expression analysis showed that NAC activated WAXY expression by binding to its promoter, indicating that NAC played a key role in starch synthesis of inferior grains under MD treatment. Our results provide new insights into the molecular mechanisms that regulate grain filling in inferior grains of rice under moderate soil drying.
机译：花后阶段施加的适度土壤干燥显着增加了劣等水稻中淀粉的积累，但尚不清楚如何在基因表达水平上调节这一过程。在这项研究中，我们在花后阶段对土壤进行了适度干燥（MD）处理，并使用RNA-seq分析跟踪了劣质谷物中可溶性糖向淀粉的转化过程的动态。 MD诱导的ABA升高水平与ABA8ox2的下调一致，这表明该基因的较低表达可能与ABA含量较高有关，可能导致劣质谷物的更好填充。此外，MD处理上调的编码5种关键酶的基因涉及蔗糖到淀粉的转化，并增加了导致劣质谷物中可溶性糖减少和淀粉积累的酶的活性。差异表达的转录因子，包括NAC，GATA，WRKY和M型MADS，预计会与其他蛋白质相互作用，介导下层籽粒对MD的响应。瞬时表达分析表明，NAC通过与其启动子结合而激活了WAXY表达，表明NAC在MD处理下在劣等谷物的淀粉合成中起关键作用。我们的结果为在中等土壤干燥条件下调节劣质水稻籽粒充实的分子机制提供了新见解。

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