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Tick Cell Lines for Study of Crimean-Congo Hemorrhagic Fever Virus and Other Arboviruses

机译:用于研究克里米亚-刚果出血热病毒和其他虫媒病毒的Line细胞系

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摘要

Continuous cell lines derived from many of the vectors of tick-borne arboviruses of medical and veterinary importance are now available. Their role as tools in arbovirus research to date is reviewed and their potential application in studies of tick cell responses to virus infection is explored, by comparison with recent progress in understanding mosquito immunity to arbovirus infection. A preliminary study of propagation of the human pathogen Crimean-Congo hemorrhagic fever virus (CCHFV) in tick cell lines is reported; CCHFV replicated in seven cell lines derived from the ticks Hyalomma anatolicum (a known vector), Amblyomma variegatum, Rhipicephalus (Boophilus) decoloratus, Rhipicephalus (Boophilus) microplus, and Ixodes ricinus, but not in three cell lines derived from Rhipicephalus appendiculatus and Ornithodoros moubata. This indicates that tick cell lines can be used to study growth of CCHFV in arthropod cells and that there may be species-specific restriction in permissive CCHFV infection at the cellular level.
机译:现已获得许多具有医学和兽医学意义的壁虱传代虫媒病毒载体的连续细胞系。通过与了解蚊子对虫媒病毒感染的最新进展进行比较,回顾了它们迄今为止在虫媒病毒研究中的工具作用,并探讨了它们在滴答细胞对病毒感染反应研究中的潜在应用。据报道,人类病原体克里米亚-刚果出血热病毒(CCHFV)在壁虱细胞系中繁殖的初步研究; CCHFV在源自an虫透明膜(已知载体),变异羊膜(Amblyomma variegatum),变色Rhipicephalus(Boophilus),colorplusus,Rhipicephalus(Boophilus)microplus和Ixodes ricinus的七个细胞系中复制,但在源自Rhipicephalus mosticus的三个细胞系中未复制。这表明tick细胞系可用于研究节肢动物细胞中CCHFV的生长,并且在细胞水平上允许的CCHFV感染可能存在物种特异性的限制。

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