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首页> 美国卫生研究院文献>Tissue Engineering. Part A >Tooth Tissue Engineering: The Importance of Blood Products as a Supplement in Tissue Culture Medium for Human Pulp Dental Stem Cells
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Tooth Tissue Engineering: The Importance of Blood Products as a Supplement in Tissue Culture Medium for Human Pulp Dental Stem Cells

机译：牙齿组织工程：血液制品作为人牙髓干细胞组织培养基中补充剂的重要性

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One of the goals in using cells for tissue engineering (TE) and cell therapy consists of optimizing the medium for cell culture. The present study compares three different blood product supplements for improved cell proliferation and protection against DNA damage in cultured human dental pulp stem cells for tooth TE applications. Human cells from dental pulp were first characterized as adult stem cells (ectomesenchymal mixed origin) by flow cytometry. Next, four different cell culture conditions were tested: I, supplement-free; II, supplemented with fetal bovine serum; III, allogeneic human serum; and IV, autologous human serum. Cultured cells were then characterized for cell proliferation, mineralized nodule formation, and colony-forming units (CFU) capability. After 28 days in culture, the comet assay was performed to assess possible damage in cellular DNA. Our results revealed that Protocol IV achieved higher cell proliferation than Protocol I (p = 0.0112). Protocols II and III resulted in higher cell proliferation than Protocol I, but no statistical differences were found relative to Protocol IV. The comet assay revealed less cell damage in cells cultured using Protocol IV as compared to Protocols II and III. The damage percentage observed on Protocol II was significantly higher than all other protocols. CFUs capability was highest using Protocol IV (p = 0.0018) and III, respectively, and the highest degree of mineralization was observed using Protocol IV as compared to Protocols II and III. Protocol IV resulted in significantly improved cell proliferation, and no cell damage was observed. These results demonstrate that human blood product supplements can be used as feasible supplements for culturing adult human dental stem cells.
机译：使用细胞进行组织工程（TE）和细胞治疗的目标之一是优化用于细胞培养的培养基。本研究比较了三种不同的血液制品补品，它们在牙齿TE应用的培养人牙髓干细胞中可改善细胞增殖并防止DNA损伤。来自牙髓的人类细胞首先通过流式细胞仪鉴定为成体干细胞（间充质混合来源）。接下来，测试了四种不同的细胞培养条件：二，补充胎牛血清；三，同种异体人血清； IV，自体人类血清。然后对培养的细胞进行细胞增殖，矿化结节形成和集落形成单位（CFU）能力的表征。培养28天后，进行彗星试验​​以评估细胞DNA中可能的损伤。我们的结果表明，方案IV比方案I具有更高的细胞增殖性（p = 0.0112）。方案II和III导致细胞增殖高于方案I，但是相对于方案IV没有发现统计学差异。与协议II和III相比，彗星试验显示使用协议IV培养的细胞中的细胞损伤更少。在方案II中观察到的损坏百分比显着高于所有其他方案。使用协议IV时CFU的能力最高（p = 0.0018）和使用III，与协议II和III相比，使用协议IV观察到的矿化度最高。方案IV导致细胞增殖显着改善，并且未观察到细胞损伤。这些结果表明，人血制品补充剂可用作培养成人人牙齿干细胞的可行补充剂。

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