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首页> 美国卫生研究院文献>Viral Immunology >A Pilot Study Comparing the Development of EIAV Env-Specific Antibodies Induced by DNA/Recombinant Vaccinia-Vectored Vaccines and an Attenuated Chinese EIAV Vaccine
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A Pilot Study Comparing the Development of EIAV Env-Specific Antibodies Induced by DNA/Recombinant Vaccinia-Vectored Vaccines and an Attenuated Chinese EIAV Vaccine

机译：DNA /重组痘苗载体疫苗和减毒的中国EIAV疫苗诱导的EIAV特异抗体开发比较的初步研究

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Data from successful attenuated lentiviral vaccine studies indicate that fully mature Env-specific antibodies characterized by high titer, high avidity, and the predominant recognition of conformational epitopes are associated with protective efficacy. Although vaccination with a DNA prime/recombinant vaccinia-vectored vaccine boost strategy has been found to be effective in some trials with non-human primate/simian/human immunodeficiency virus (SHIV) models, it remains unclear whether this vaccination strategy could elicit mature equine infectious anemia virus (EIAV) Env-specific antibodies, thus protecting vaccinated horses against EIAV infection. Therefore, in this pilot study we vaccinated horses using a strategy based on DNA prime/recombinant Tiantan vaccinia (rTTV)-vectored vaccines encoding EIAV env and gag genes, and observed the development of Env-specific antibodies, neutralizing antibodies, and p26-specific antibodies. Vaccination with DNA induced low titer, low avidity, and the predominant recognition of linear epitopes by Env-specific antibodies, which was enhanced by boosting vaccinations with rTTV vaccines. However, the maturation levels of Env-specific antibodies induced by the DNA/rTTV vaccines were significantly lower than those induced by the attenuated vaccine EIAVFDDV. Additionally, DNA/rTTV vaccines did not elicit broadly neutralizing antibodies. After challenge with a virulent EIAV strain, all of the vaccinees and control horses died from EIAV disease. These data indicate that the regimen of DNA prime/rTTV vaccine boost did not induce mature Env-specific antibodies, which might have contributed to immune protection failure.
机译：成功的减毒慢病毒疫苗研究数据表明，具有高滴度，高亲和力和构象表位优势的特征的完全成熟的Env特异性抗体与保护功效相关。尽管在一些使用非人类灵长类/猿猴/人类免疫缺陷病毒（SHIV）模型的试验中发现了用DNA初免/重组牛痘载体疫苗加强策略进行疫苗接种是有效的，但仍不清楚这种疫苗接种策略是否可以引发成熟的马传染性贫血病毒（EIAV）Env特异性抗体，从而保护接种疫苗的马匹免受EIAV感染。因此，在这项前瞻性研究中，我们使用了基于EIAV env和gag基因的DNA原初/重组天坛牛痘（rTTV）载体疫苗的策略对马进行了疫苗接种，并观察了Env特异性抗体，中和抗体和p26特异性抗体的发展抗体。用DNA疫苗接种可诱导低滴度，低亲和力，并且主要通过Env特异性抗体识别线性表位，而通过rTTV疫苗加强疫苗接种可增强这种免疫力。但是，DNA / rTTV疫苗诱导的Env特异性抗体的成熟水平显着低于减毒疫苗EIAVFDDV诱导的Env特异性抗体的成熟水平。此外，DNA / rTTV疫苗未引起广泛的中和抗体。用强毒EIAV株攻击后，所有疫苗接种者和对照马都死于EIAV疾病。这些数据表明，DNA初免/ rTTV疫苗加强方案未诱导成熟的Env特异性抗体，这可能导致免疫保护失败。

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