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首页> 美国卫生研究院文献>Oncology Letters >Matrigel induces L-plastin expression and promotes L-plastin-dependent invasion in human cholangiocarcinoma cells
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Matrigel induces L-plastin expression and promotes L-plastin-dependent invasion in human cholangiocarcinoma cells

机译：基质胶诱导人胆管癌细胞中L-塑蛋白表达并促进L-塑蛋白依赖性侵袭

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The function of the extracellular matrix (ECM) in the tumor microenvironment is not limited to forming a barrier against tumor invasion. As demonstrated in pathological specimens, cholangiocarcinoma samples exhibit an enrichment of the ECM surrounding the tumor cells. In this study, we examined involvement of the ECM in the regulation of the invasiveness of cholangiocarcinoma cells. The RMCCA1 cholangiocarcinoma cell line was cultured in culture plates either with or without a coating of reconstituted ECM basement membrane preparation (BD Matrigel matrix). In vitro invasion assays were then performed. In addition, the protein expression profile of the cell line was examined using two-dimensional gel electrophoresis and liquid chromatography-tandem mass spectrometry. The proteins expressed and their functional associations with cancer progression were determined. Culturing the RMCCA1 cell line in the BD Matrigel matrix induced cell invasion. Numerous proteins were induced by culturing the RMCCA1 cells in the matrix gel. The expression of L-plastin, an actin-binding protein, was significantly upregulated. The knockdown of L-plastin expression by siRNA silencing significantly suppressed the cellular response to matrix gel-stimulated cancer cell invasion. The ECM promotes the invasiveness of cholangiocarcinoma cells by upregulating L-plastin. These findings suggest the potential exploitation of this mechanism as a means of inhibiting the invasiveness of cholangiocarcinoma cells.
机译：肿瘤微环境中细胞外基质（ECM）的功能不限于形成抵抗肿瘤侵袭的屏障。如病理标本所示，胆管癌样品在肿瘤细胞周围表现出丰富的ECM。在这项研究中，我们检查了ECM在胆管癌细胞侵袭性调节中的参与。将RMCCA1胆管癌细胞系培养在有或没有重组ECM基膜制剂（BD Matrigel基质）涂层的培养板上。然后进行体外侵袭测定。另外，使用二维凝胶电泳和液相色谱-串联质谱法检查细胞系的蛋白质表达谱。确定表达的蛋白质及其与癌症进展的功能关联。在BD Matrigel基质中培养RMCCA1细胞系可诱导细胞侵袭。通过在基质凝胶中培养RMCCA1细胞来诱导许多蛋白质。肌动蛋白结合蛋白L-plastin的表达明显上调。 siRNA沉默可抑制L-plastin表达，从而显着抑制细胞对基质凝胶刺激的癌细胞侵袭的反应。 ECM通过上调L-增塑素来促进胆管癌细胞的侵袭性。这些发现表明该机制作为抑制胆管癌细胞侵袭性的手段的潜在利用。

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