• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Oncology Letters >MicroRNA-92 regulates cervical tumorigenesis and its expression is upregulated by human papillomavirus-16 E6 in cervical cancer cells
【2h】

MicroRNA-92 regulates cervical tumorigenesis and its expression is upregulated by human papillomavirus-16 E6 in cervical cancer cells

机译：MicroRNA-92调节子宫颈癌的发生其表达被人乳头瘤病毒-16 E6在子宫颈癌细胞中上调

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

MicroRNA (miR)-92 is overexpressed in a number of tumors and has been proven to negatively regulate a number of tumor suppressor genes, including phosphatase and tensin homologue (PTEN). However, its function and molecular mechanism(s) of action in squamous cervical carcinoma (SCCs) have not been well described. Furthermore, the correlation between miR-92 and human papillomavirus (HPV)-16 E6 has not been studied. In the present study, miR-92 expression levels were quantified using quantitative PCR (qPCR) in cervical cancer tissues, normal cervical tissues and cervical cancer cell lines. SiHa cells were transfected with either miR-92-mimics, anti-miR-92 or negative controls. C33A cells were stably transfected with pEGFP-N1-16E6 and pEGFP-N1-neo plasmids. The levels of PTEN protein expression in the transfected SiHa and C33A cells were evaluated using western blot analysis. The effects of miR-92 were detected using cell counting kit (CCK)-8 and Transwell assays. HPV16 E6 siRNA was used to detect the effectiveness of the E6 protein on miR-92 in the SiHa and C33A cells. miR-92 was highly-expressed in the human cervical cancer tissues compared with the normal tissues. In the HPV16-positive cervical cancer tissues, the expression of miR-92 was higher compared with the HPV16-negative cervical cancer tissues. HPV16 E6 upregulated miR-92 expression in the SiHa- and C33A-pEGFP-N1-16E6 cells. The upregulation of miR-92 promoted cell growth and invasion in the SiHa cells. PTEN protein expression was decreased in the SiHa cells that were transfected with the miR-92 mimic. The data indicated that miR-92 may increase the migration and invasion of SiHa cells, partially through the downregulation of PTEN protein expression. HPV16 E6 was identified to upregulate miR-92 expression.
机译：MicroRNA（miR）-92在许多肿瘤中均过表达，并已被证明可负调控许多肿瘤抑制基因，包括磷酸酶和张力蛋白同源物（PTEN）。然而，其在鳞状宫颈癌（SCC）中的功能和作用的分子机制尚未得到很好的描述。此外，尚未研究miR-92与人乳头瘤病毒（HPV）-16 E6之间的相关性。在本研究中，使用定量PCR（qPCR）对子宫颈癌组织，正常子宫颈组织和子宫颈癌细胞系中的miR-92表达水平进行了定量。用miR-92-模拟物，抗miR-92或阴性对照转染SiHa细胞。用pEGFP-N1-16E6和pEGFP-N1-neo质粒稳定转染C33A细胞。使用蛋白质印迹分析评估了转染的SiHa和C33A细胞中PTEN蛋白的表达水平。使用细胞计数试剂盒（CCK）-8和Transwell测定法检测miR-92的作用。 HPV16 E6 siRNA用于检测SiHa和C33A细胞中E6蛋白对miR-92的有效性。与正常组织相比，miR-92在人宫颈癌组织中高表达。在HPV16阳性子宫颈癌组织中，与HPV16阴性子宫颈癌组织相比，miR-92的表达更高。 HPV16 E6上调了SiHa-和C33A-pEGFP-N1-16E6细胞中的miR-92表达。 miR-92的上调促进了SiHa细胞中的细胞生长和侵袭。在用miR-92模拟物转染的SiHa细胞中，PTEN蛋白表达降低。数据表明，miR-92可能部分下调PTEN蛋白表达，从而增加SiHa细胞的迁移和侵袭。鉴定出HPV16 E6上调miR-92表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号