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首页> 美国卫生研究院文献>Scientific Reports >Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound
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Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound

机译：冷物理等离子体的化学指纹图–使用半胱氨酸作为示踪化合物的实验和计算研究

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Reactive oxygen and nitrogen species released by cold physical plasma are being proposed as effectors in various clinical conditions connected to inflammatory processes. As these plasmas can be tailored in a wide range, models to compare and control their biochemical footprint are desired to infer on the molecular mechanisms underlying the observed effects and to enable the discrimination between different plasma sources. Here, an improved model to trace short-lived reactive species is presented. Using FTIR, high-resolution mass spectrometry, and molecular dynamics computational simulation, covalent modifications of cysteine treated with different plasmas were deciphered and the respective product pattern used to generate a fingerprint of each plasma source. Such, our experimental model allows a fast and reliable grading of the chemical potential of plasmas used for medical purposes. Major reaction products were identified to be cysteine sulfonic acid, cystine, and cysteine fragments. Less-abundant products, such as oxidized cystine derivatives or S-nitrosylated cysteines, were unique to different plasma sources or operating conditions. The data collected point at hydroxyl radicals, atomic O, and singlet oxygen as major contributing species that enable an impact on cellular thiol groups when applying cold plasma in vitro or in vivo.
机译：由冷的物理等离子体释放的活性氧和氮物质被提议作为与炎症过程相关的各种临床条件的效应器。由于这些血浆可以在很宽的范围内定制，因此需要模型来比较和控制其生化足迹，以推断观察到的效应背后的分子机制，并能够区分不同血浆来源。在这里，提出了一种跟踪短期反应物种的改进模型。使用FTIR，高分辨率质谱和分子动力学计算模拟，对用不同血浆处理的半胱氨酸的共价修饰进行了解密，并将各自的产物模式用于生成每个血浆源的指纹。这样，我们的实验模型可以对用于医疗目的的血浆的化学势进行快速而可靠的分级。鉴定出主要反应产物为半胱氨酸磺酸，胱氨酸和半胱氨酸片段。含量较低的产品，例如氧化的胱氨酸衍生物或S-亚硝化的半胱氨酸，对于不同的血浆来源或操作条件而言是独特的。收集到的数据指向羟基自由基，原子O和单线态氧，这是主要的贡献物质，当在体外或体内应用冷血浆时，它们会对细胞硫醇基产生影响。

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