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首页> 美国卫生研究院文献>Scientific Reports >Chronic rapamycin treatment on the nutrient utilization and metabolism of juvenile turbot (Psetta maxima)
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Chronic rapamycin treatment on the nutrient utilization and metabolism of juvenile turbot (Psetta maxima)

机译：慢性雷帕霉素处理对大菱turbo营养利用和代谢的影响

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摘要

High dietary protein inclusion is necessary in fish feeds and also represents a major cost in the aquaculture industry, which demands improved dietary conversion into body proteins in fish. In mammals, the target of rapamycin (TOR) is a key nutritionally responsive molecule governing postprandial anabolism. However, its physiological significance in teleosts has not been fully examined. In the present study, we examined the nutritional physiology of turbot after chronic rapamycin inhibition. Our results showed that a 6-week inhibition of TOR using dietary rapamycin inclusion (30 mg/kg diet) reduced growth performance and feed utilization. The rapamycin treatment inhibited TOR signaling and reduced expression of key enzymes in glycolysis, lipogenesis, cholesterol biosynthesis, while increasing the expression of enzymes involved in gluconeogenesis. Furthermore, rapamycin treatment increased intestinal goblet cell number in turbot, while the expressions of Notch and Hes1 were down regulated. It was possible that stimulated goblet cell differentiation by rapamycin was mediated through Notch-Hes1 pathway. Therefore, our results demonstrate the important role of TOR signaling in fish nutritional physiology.
机译：鱼饲料中必须富含膳食蛋白质，这也是水产养殖业的主要成本，水产养殖业需要提高饲料中鱼类向人体蛋白质的饮食转化率。在哺乳动物中，雷帕霉素（TOR）的靶标是控制餐后合成代谢的关键营养反应分子。然而，其在硬骨鱼中的生理意义尚未得到充分研究。在本研究中，我们检查了慢性雷帕霉素抑制后大菱t的营养生理。我们的结果表明，使用雷帕霉素饮食（30μmg/ kg日粮）对TOR抑制6周会降低生长性能和饲料利用率。雷帕霉素治疗可抑制TOR信号转导，并降低糖酵解，脂肪生成，胆固醇生物合成中关键酶的表达，同时增加参与糖异生的酶的表达。此外，雷帕霉素处理增加了大菱t中肠杯状细胞的数量，同时Notch和Hes1的表达下调。雷帕霉素刺激的杯状细胞分化可能是通过Notch-Hes1途径介导的。因此，我们的结果证明了TOR信号在鱼类营养生理中的重要作用。

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