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首页> 美国卫生研究院文献>Scientific Reports >Germ plasm localisation of the HELICc of Vasa in Drosophila: analysis of domain sufficiency and amino acids critical for localisation
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Germ plasm localisation of the HELICc of Vasa in Drosophila: analysis of domain sufficiency and amino acids critical for localisation

机译：果蝇中瓦萨HELICc的种质定位：结构域充足性和对定位至关重要的氨基酸的分析

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Formation of the germ plasm drives germline specification in Drosophila and some other insects such as aphids. Identification of the DEAD-box protein Vasa (Vas) as a conserved germline marker in flies and aphids suggests that they share common components for assembling the germ plasm. However, to which extent the assembly order is conserved and the correlation between functions and sequences of Vas remain unclear. Ectopic expression of the pea aphid Vas (ApVas1) in Drosophila did not drive its localisation to the germ plasm, but ApVas1 with a replaced C-terminal domain (HELICc) of Drosophila Vas (DmVas) became germ-plasm restricted. We found that HELICc itself, through the interaction with Oskar (Osk), was sufficient for germ-plasm localisation. Similarly, HELICc of the grasshopper Vas could be recruited to the germ plasm in Drosophila. Nonetheless, germ-plasm localisation was not seen in the Drosophila oocytes expressing HELICcs of Vas orthologues from aphids, crickets, and mice. We further identified that glutamine (Gln) 527 within HELICc of DmVas was critical for localisation, and its corresponding residue could also be detected in grasshopper Vas yet missing in the other three species. This suggests that Gln527 is a direct target of Osk or critical to the maintenance of HELICc conformation.
机译：种质的形成驱动果蝇和其他一些昆虫（如蚜虫）的种系规格。 DEAD-box蛋白瓦萨（Vas）作为果蝇和蚜虫中保守的种系标志物的鉴定表明，它们具有组装种质的共同成分。但是，在多大程度上保留了组装顺序，并且Vas的功能和序列之间的相关性仍然不清楚。豌豆蚜虫Vas（ApVas1）在果蝇中的异位表达不会推动其定位到种质，但是果蝇Vas（DmVas）的C末端结构域（HELICc）被替换的ApVas1成为种质限制。我们发现，通过与Oskar（Osk）的相互作用，HELICc本身足以进行种质定位。同样，蚱Va Vas的HELICc可以募集到果蝇的种质。尽管如此，在表达来自蚜虫，和小鼠的Vas直系同源物的HELICc的果蝇卵母细胞中未观察到种质定位。我们进一步确定，DmVas的HELICc中的谷氨酰胺（Gln）527对定位至关重要，在蝗虫Vas中也可以检测到其相应的残留物，而在其他三个物种中却没有。这表明Gln527是Osk的直接靶标或对维持HELICc构象至关重要。

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