• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Scientific Reports >Identification and profiling of microRNAs during gonadal development in the giant freshwater prawn Macrobrachium rosenbergii
【2h】

Identification and profiling of microRNAs during gonadal development in the giant freshwater prawn Macrobrachium rosenbergii

机译：罗氏沼虾巨大性腺性腺发育过程中microRNA的鉴定和分析。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

As post-transcriptional regulators, microRNAs (miRNAs) play an important role in growth and reproductive processes. So far, there is limited information regarding crustacean miRNAs. To explore the potential role of miRNAs in the gonadal development of the prawn Macrobrachium rosenbergii, we constructed seven small RNA libraries from ovarian and testicular tissues at various stages using somatic tissue as the control. A total of 1,954 known and 129 novel miRNAs were retrieved. By comparing differentially expressed miRNAs (DEMs) between testes and ovaries, forty-one miRNAs were identified with sex-biased expression patterns, including 17 ovary-biased and 24 testis-biased patterns. Furthermore, the putative target genes of the sex-biased miRNAs, such as cyclin L1, mitogen-activated protein kinase 7 (MAPK 7), heat shock protein (HSP), and zinc finger protein, were significantly enriched in many reproduction-related pathways including the Gonadotropin-releasing hormone (GnRH) pathway, glycolysis, gluconeogenesis pathway, ovarian steroidogenesis, estrogen signaling pathway, MAPK pathway, Wnt pathway, and insulin signaling pathway, implicating potential regulatory roles of miRNAs in reproduction. These data aid in the further investigation of the mechanism of gonadal development and reproductive regulation mediated by miRNA in M. rosenbergii.
机译：作为转录后调节剂，微小RNA（miRNA）在生长和生殖过程中起重要作用。迄今为止，关于甲壳类miRNA的信息有限。为了探索miRNA在对虾罗氏沼虾的性腺发育中的潜在作用，我们使用体细胞组织作为对照，从卵巢和睾丸组织的各个阶段构建了七个小RNA库。总共检索了1,954个已知的miRNA和129个新颖的miRNA。通过比较睾丸和卵巢之间差异表达的miRNA（DEM），鉴定出41个具有性别偏向的表达模式的miRNA，包括17个卵巢偏向和24个睾丸偏向的模式。此外，性别偏见的miRNA的假定靶基因，例如细胞周期蛋白L1，丝裂原激活的蛋白激酶7（MAPK 7），热休克蛋白（HSP）和锌指蛋白，在许多与繁殖有关的途径中均得到了丰富的表达。包括促性腺激素释放激素（GnRH）途径，糖酵解，糖异生途径，卵巢类固醇生成，雌激素信号途径，MAPK途径，Wnt途径和胰岛素信号途径，这暗示了miRNA在生殖中的潜在调控作用。这些数据有助于进一步研究罗氏沼虾中miRNA介导的性腺发育和生殖调控机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号