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A METHOD OF IDENTIFYING PARENTAGE IN FRESHWATER PRAWN MACROBRACHIUM ROSENBERGII
A METHOD OF IDENTIFYING PARENTAGE IN FRESHWATER PRAWN MACROBRACHIUM ROSENBERGII
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机译：淡水虾罗氏沼虾的亲本鉴定方法
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摘要
A method of identifying parentage in Macrobrachium rosenbergii prawn comprising the steps of amplifying targeted EST-SSR nucleic acid sequences from a biological sample of Macrobrachium rosenbergii through a polymerase chain reaction using primer pairs with nucleic acid sequences as set forth in SEQ ID NO. 1 and SEQ ID NO. 2, SEQ ID NO. 3 and SEQ ID NO. 4, SEQ ID NO. 5 and SEQ ID NO. 6, SEQ ID NO. 7 and SEQ ID NO. 8, SEQ ID NO. 9 and SEQ ID NO. 10, SEQ ID NO. 11 and SEQ ID NO. 12 to produce respective amplicons; determining fragment size of the produced amplicons; regarding the fragment size of the amplicons corresponding to predetermined alleles; and identifying possible parentage of the prawn based on the regarded allele.
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机译：一种在罗氏沼虾虾中鉴定亲本的方法，该方法包括以下步骤：使用具有SEQ ID NO.1所示核酸序列的引物对，通过聚合酶链反应从罗氏沼虾的生物样品中扩增靶向的EST-SSR核酸序列。 1和SEQ ID NO。 SEQ ID NO.2， 3和SEQ ID NO。 4，SEQ ID NO。 5和SEQ ID NO。 6，SEQ ID NO。 7和SEQ ID NO。 SEQ ID NO.8， 9和SEQ ID NO。 10，SEQ ID NO。 11和SEQ ID NO。 12个产生各自的扩增子;确定产生的扩增子的片段大小;关于对应于预定等位基因的扩增子的片段大小;并根据所考虑的等位基因确定对虾的可能亲缘关系。
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