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Toxicological and biochemical responses of the earthworm Eisenia fetida to cyanobacteria toxins

机译:EEisenia fetida对蓝细菌毒素的毒理学和生化反应

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摘要

Irrigation with eutrophic water containing cyanobacteria toxins poses a potential risk to soil animals. To evaluate ecotoxicological effect of microcystins (MCs) on earthworms, filter paper acute toxicity test, avoidance test and a 14-d artificial soil test were carried out. No acute toxicity was found in the filter paper test, and earthworms showed no avoidance response to MCs exposure. In the artificial soil test, Eisenia fetida were allowed to grow in presence or absence of MCs (0, 1, 10, 100, 1000 μg kg−1 of soil) for 1, 7, and 14 d. Results showed that MCs could bioaccumulated in earthworm. A stimulatory effect on catalase and glutathione oxidase activities induced by MCs was found on day 1, and both of them were significantly inhibited at 100 and 1000 μg kg−1 on days 14. The superoxide dismutase activity was relatively insensitive. Significant increase of malondialdehyde content and decrease of neutral red retention time were observed at 100 and 1000 μg kg−1 on days 7 and 14. Our results suggest that MCs induces oxidative stress on earthworms, which leads to disruption of the antioxidant system and lipid peroxidation, as well as alterations in lysosomal membrane stability.
机译:用含有蓝细菌毒素的富营养水灌溉对土壤动物构成潜在风险。为了评价微囊藻毒素(MCs)对earth的生态毒理作用,进行了滤纸急性毒性试验,回避试验和14天人工土壤试验。在滤纸测试中未发现急性毒性,并且showed对MCs暴露没有回避反应。在人工土壤试验中,允许费氏埃塞氏菌在有或没有MCs(0、1、10、100、1000μg kg -1 土壤)存在下生长1、7和14 d 。结果表明,MCs可以在worm中生物富集。在第1天发现对MCs诱导的过氧化氢酶和谷胱甘肽氧化酶活性有刺激作用,并且在第14天分别在100和1000μgkg -1 时均被显着抑制。超氧化物歧化酶活性相对较高不敏感。在第7天和第14天,在100和1000μgkg -1 时,丙二醛含量显着增加,中性红色保留时间减少。抗氧化系统和脂质过氧化作用,以及溶酶体膜稳定性的改变。

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