• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Scientific Reports >Novelty application of multi-omics correlation in the discrimination of sulfur-fumigation and non-sulfur-fumigation Ophiopogonis Radix
【2h】

Novelty application of multi-omics correlation in the discrimination of sulfur-fumigation and non-sulfur-fumigation Ophiopogonis Radix

机译：多元组学相关性在硫熏和非硫熏麦田鉴别中的新颖应用

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A rapid and sensitive approach to differentiate sulfur-fumigated (SF) Ophiopogonis Radix based on Multi-Omics Correlation Analysis (MOCA) strategy was first established. It was characterized by multiple data-acquisition methods (NIR, HPLC, and UHPLC-HRMS) based metabonomics and multivariate statistical analysis methods. As a result, SF and non-sulfur fumigated (NSF) Ophiopogonis Radix samples were efficaciously discriminated. Moreover, based on the acquired HRMS data, 38 sulfur-containing discriminatory markers were eventually characterized, whose NIR absorption could be in close correlation with the discriminatory NIR wavebands (5000–5200 cm−1) screened by NIR metabonomics coupled with SiPLS and 2D-COS methods. This results were also validated from multiple perspectives, including metabonomics analysis based on the discriminatory markers and the simulation of SF ophiopogonin D and Ophiopogonis Radix sample. In conclusion, our results first revealed the intrinsic mechanism of discriminatory NIR wavebands by means of UHPLC-HRMS analysis. Meanwhile, the established MOCA strategy also provided a promising NIR based differential method for SF Ophiopogonis Radix, which could be exemplary for future researches on rapid discrimination of other SF Chinese herbal medicines.
机译：首先建立了一种基于多组相关分析（MOCA）策略的快速灵敏的硫熏烟麦草根鉴别方法。它的特征在于基于代谢组学的多种数据采集方法（NIR，HPLC和UHPLC-HRMS）和多元统计分析方法。结果，有效地区分了SF和非硫熏蒸的麦草根样品。此外，根据获得的HRMS数据，最终鉴定了38个含硫的鉴别标记，其NIR吸收可能与NIR筛选出的鉴别性NIR波段（5000-5200 cm -1 ）密切相关。代谢组学与SiPLS和2D-COS方法结合使用。还从多个角度验证了该结果，包括基于区分性标记的代谢组学分析以及对SF麦冬色素D和麦冬样品的模拟。总之，我们的结果首先通过UHPLC-HRMS分析揭示了可辨别NIR波段的内在机理。同时，既定的MOCA策略也为顺丁麦冬的鉴别提供了一种基于NIR的有前途的鉴别方法，可以作为今后对其他SF中草药快速鉴别的研究的范例。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号