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Two hAT transposon genes were transferred from Brassicaceae to broomrapes and are actively expressed in some recipients

机译:两个hAT转座子基因从十字花科转移到扫帚上并在某些受体中活跃表达

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摘要

A growing body of evidence is pointing to an important role of horizontal gene transfer (HGT) in the evolution of higher plants. However, reports of HGTs of transposable elements (TEs) in plants are still scarce, and only one case is known of a class II transposon horizontally transferred between grasses. To investigate possible TE transfers in dicots, we performed transcriptome screening in the obligate root parasite Phelipanche aegyptiaca (Orobanchaceae), data-mining in the draft genome assemblies of four other Orobanchaceae, gene cloning, gene annotation in species with genomic information, and a molecular phylogenetic analysis. We discovered that the broomrape genera Phelipanche and Orobanche acquired two related nuclear genes (christened BO transposase genes), a new group of the hAT superfamily of class II transposons, from Asian Sisymbrieae or a closely related tribe of Brassicaceae, by HGT. The collinearity of the flanking genes, lack of a classic border structure, and low expression levels suggest that BO transposase genes cannot transpose in Brassicaceae, whereas they are highly expressed in P. aegyptiaca.
机译:越来越多的证据表明,水平基因转移(HGT)在高等植物的进化中具有重要作用。然而,关于植物中转座因子(TEs)的HGT的报道仍然很少,仅一种情况已知在草之间水平转移的II类转座子。为了研究双子叶植物中可能的TE转移,我们在专性根寄生虫Phelipanche aegyptiaca(Orobanchaceae)中进行了转录组筛选,在另外四个Orobanchaceae的基因组草图中进行了数据挖掘,基因克隆,具有基因组信息的物种中的基因注释和分子系统发育分析。我们发现扫帚rap属Phelipanche和Orobanche属由HGT从亚洲Sisymbrieae或十字花科的一个密切相关的部落中获得了两个相关的核基因(被称为BO转座酶基因),即一组II类转座子的hAT超家族的新成员。侧翼基因的共线性,缺乏经典的边界结构和低表达水平表明BO转座酶基因不能在十字花科中转座,而在紫菜中则高表达。

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