• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Scientific Reports >Identification of novel and robust internal control genes from Volvariella volvacea that are suitable for RT-qPCR in filamentous fungi
【2h】

Identification of novel and robust internal control genes from Volvariella volvacea that are suitable for RT-qPCR in filamentous fungi

机译：鉴定了适用于丝状真菌RT-qPCR的Volvolvella volvacea新颖而强大的内部控制基因

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The selection of appropriate internal control genes (ICGs) is a crucial step in the normalization of real-time quantitative PCR (RT-qPCR) data. Housekeeping genes are habitually selected for this purpose, despite accumulating evidence on their instability. We screened for novel, robust ICGs in the mushroom forming fungus Volvariella volvacea. Nine commonly used and five newly selected ICGs were evaluated for expression stability using RT-qPCR data in eight different stages of the life cycle of V. volvacea. Three different algorithms consistently determined that three novel ICGs (SPRYp, Ras and Vps26) exhibited the highest expression stability in V. volvacea. Subsequent analysis of ICGs in twenty-four expression profiles from nine filamentous fungi revealed that Ras was the most stable ICG amongst the Basidiomycetous samples, followed by SPRYp, Vps26 and ACTB. Vps26 was expressed most stably within the analyzed data of Ascomycetes, followed by HH3 and β-TUB. No ICG was universally stable for all fungal species, or for all experimental conditions within a species. Ultimately, the choice of an ICG will depend on a specific set of experiments. This study provides novel, robust ICGs for Basidiomycetes and Ascomycetes. Together with the presented guiding principles, this enables the efficient selection of suitable ICGs for RT-qPCR.
机译：适当的内部控制基因（ICG）的选择是实时定量PCR（RT-qPCR）数据标准化的关键步骤。尽管有证据表明管家基因不稳定，但仍习惯性地为此选择管家基因。我们筛选了蘑菇形成菌沃尔沃菌丝菌中的新型，稳定的ICG。使用逆转录聚合酶链反应PCR数据，在菌丝藻生命周期的八个不同阶段中评估了九种常用ICG和五种新选择的ICG的表达稳定性。三种不同的算法一致地确定了三种新颖的ICG（SPRYp，Ras和Vps26）在volv。volvacea中表现出最高的表达稳定性。随后对来自9种丝状真菌的24种表达谱中的ICG进行分析，结果表明，Ras是芽孢杆菌样品中最稳定的ICG，其次是SPRYp，Vps26和ACTB。在子囊菌的分析数据中，Vps26最稳定地表达，其次是HH3和β-TUB。对于所有真菌物种，或物种内的所有实验条件，没有ICG普遍稳定。最终，ICG的选择将取决于一组特定的实验。这项研究为担子菌和子囊菌提供了新颖，强大的ICG。结合提出的指导原则，这使得可以为RT-qPCR有效选择合适的ICG。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号