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Comparative transcriptome analysis reveals potential fruiting body formation mechanisms in Morchella importuna

机译：比较转录组分析揭示了羊肚菌中潜在的子实体形成机制

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摘要

Morchella importuna has been artificially cultivated, but stable production remains difficult because its mechanisms of fruiting body formation are unclear. To investigate the fruiting body formation mechanisms, we sequenced the transcriptomes of Morchella importuna at the mycelial and young fruiting body stages. Among the 12,561 differentially expressed genes (DEGs), 9215 were upregulated, and 3346 were downregulated. DEG enrichment analysis showed that these genes were enriched in the “generation of precursor metabolites and energy”, “carbohydrate catabolic process”, and “oxidoreductase activity” Gene Ontology (GO) functional categories. Enzyme activity assay results indicated that the activity levels of CAZymes (carbohydrate-active enzymes), oxidoreductases (SOD (superoxide dismutase), CAT (catalase)) and mitochondrial complex (complex I, II, III) proteins were significantly increased from the mycelial stage to the young fruiting body stage. In addition, the genes encoding CAZymes, mitochondrial proteins, oxidoreductases and heat shock proteins had higher expression levels in the young fruiting body stage than in the mycelial stage, and the qRT-PCR results showed similar trends to the RNA-Seq results. In summary, these results suggest that carbohydrate catabolism and energy metabolism are significantly enhanced in the young fruiting body stage and that growth environment temperature changes affect the formation of fruiting bodies.Electronic supplementary materialThe online version of this article (10.1186/s13568-019-0831-4) contains supplementary material, which is available to authorized users.
机译：羊肚菌已被人工栽培，但由于其子实体形成的机制尚不清楚，因此稳定生产仍然很困难。为了研究子实体的形成机理，我们在菌丝体和子实体期对了羊肚菌的转录组进行了测序。在12561个差异表达基因（DEG）中，有9215个上调，有3346个下调。 DEG富集分析表明，这些基因在“前体代谢物和能量的产生”，“碳水化合物分解代谢过程”和“氧化还原酶活性”基因本体论（GO）功能类别中得到了丰富。酶活性测定结果表明，从菌丝体阶段开始，CAZymes（碳水化合物活性酶），氧化还原酶（SOD（超氧化物歧化酶），CAT（过氧化氢酶））和线粒体复合体（复合体I，II，III）的活性水平显着提高。进入年轻的子实体阶段。此外，编码CAZymes，线粒体蛋白，氧化还原酶和热休克蛋白的基因在幼果期比在菌丝期具有更高的表达水平，qRT-PCR结果显示出与RNA-Seq结果相似的趋势。总之，这些结果表明，在年轻子实体阶段，碳水化合物的分解代谢和能量代谢显着增强，并且生长环境温度的变化会影响子实体的形成。电子补充材料本文的在线版本（10.1186 / s13568-019-0831 -4）包含补充材料，授权用户可以使用。

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