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首页> 美国卫生研究院文献>Nucleic Acids Research >The general mRNA exporters Mex67 and Mtr2 play distinct roles in nuclear export of tRNAs in Trypanosoma brucei
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The general mRNA exporters Mex67 and Mtr2 play distinct roles in nuclear export of tRNAs in Trypanosoma brucei

机译：一般的mRNA出口子Mex67和Mtr2在布鲁氏锥虫中tRNA的核出口中起不同的作用

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摘要

Transfer RNAs (tRNAs) are central players in protein synthesis, which in Eukarya need to be delivered from the nucleus to the cytoplasm by specific transport receptors, most of which belong to the evolutionarily conserved beta-importin family. Based on the available literature, we identified two candidates, Xpo-t and Xpo-5 for tRNA export in Trypanosoma brucei. However, down-regulation of expression of these genes did not disrupt the export of tRNAs to the cytoplasm. In search of alternative pathways, we tested the mRNA export complex Mex67-Mtr2, for a role in tRNA nuclear export, as described previously in yeast. Down-regulation of either exporter affected the subcellular distribution of tRNAs. However, contrary to yeast, TbMex67 and TbMtr2 accumulated different subsets of tRNAs in the nucleus. While TbMtr2 perturbed the export of all the tRNAs tested, silencing of TbMex67, led to the nuclear accumulation of tRNAs that are typically modified with queuosine. In turn, inhibition of tRNA nuclear export also affected the levels of queuosine modification in tRNAs. Taken together, the results presented demonstrate the dynamic nature of tRNA trafficking in T. brucei and its potential impact not only on the availability of tRNAs for protein synthesis but also on their modification status.
机译：转移RNA（tRNA）是蛋白质合成中的核心角色，在Eukarya中，需要通过特定的转运受体将其从核中传递到细胞质，其中大多数属于进化上保守的β-importin家族。根据现有文献，我们确定了两个候选物，Xpo-t和Xpo-5，用于布氏锥虫的tRNA出口。但是，这些基因表达的下调并没有破坏tRNA向细胞质的输出。为了寻找替代途径，我们测试了mRNA出口复合物Mex67-Mtr2在tRNA核出口中的作用，如先前在酵母中所述。任一个出口商的下调影响了tRNA的亚细胞分布。但是，与酵母相反，TbMex67和TbMtr2在细胞核中积累了不同的tRNA亚集。尽管TbMtr2干扰了所有测试的tRNA的输出，但TbMex67的沉默导致了通常用queussine修饰的tRNA的核积累。反过来，对tRNA核输出的抑制也影响了tRNA中Queusine修饰的水平。两者合计，提出的结果证明了t.brucei中tRNA交易的动态性质及其潜在影响，不仅影响tRNA的蛋白质合成可用性，而且还影响其修饰状态。

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