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首页> 美国卫生研究院文献>Regenerative Biomaterials >Tissue engineering using 3D printed nano-bioactive glass loaded with NELL1 gene for repairing alveolar bone defects
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Tissue engineering using 3D printed nano-bioactive glass loaded with NELL1 gene for repairing alveolar bone defects

机译：使用装有NELL1基因的3D打印纳米生物活性玻璃进行组织工程修复牙槽骨缺损

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摘要

The purposes of this study were to construct a novel tissue engineered bone composed of 3D-printed bioactive glass block/chitosan nanoparticles (BD/CSn) composites loaded with Nel-like Type I molecular-1 DNA (pDNA-NELL1) and/or bone marrow mesenchymal stem cells (BMSCs), and study their osteogenic activities by repairing bone defects in rhesus monkeys. CSn with NELL1 gene plasmid and rhesus monkey BMSCs were composited with a BD scaffold to prepare the tissue-engineered bone. Four adult female rhesus monkeys with 10- to 12-years old and 5–7 kg in weight were used in animal experiments. The first and second premolar teeth from four regions of each monkey were removed to form bone defects with size of 10 × 10 × 5 mm, which were then implanted with above-mentioned tissue engineered bone. At 12 weeks after the implantation, gross observations, X-ray and micro-CT observations revealed that the new bone was extremely close to normal bone in mass, density, hardness, and structure. The bony cortex was smooth and closely connected to the surrounding normal bone. Histological observations revealed moderate inflammation in the repair area, and the new bone tissues were similar to normal ones. In conclusion, tissue engineered bone of this study exhibited good osteoconductivity for promoting the formation of new alveolar bone tissue, and NELL1 gene played a promotional role in bone regeneration.
机译：这项研究的目的是构建由3D打印的生物活性玻璃块/壳聚糖纳米颗粒（BD / CSn）复合材料组成的新型组织工程骨，该复合材料上装有Nel样的I型第1分子1 DNA（pDNA-NELL1）和/或骨骼骨髓间充质干细胞（BMSC），并通过修复恒河猴的骨缺损来研究其成骨活性。将具有NELL1基因质粒的CSn和恒河猴BMSC与BD支架复合，以制备组织工程骨。在动物实验中使用了四只成年雌性恒河猴，它们的年龄为10至12岁，体重为5-7 kg。去除每只猴子四个区域的第一和第二磨牙，形成大小为10×10×5 mm的骨缺损，然后将其植入上述组织工程化骨中。植入后第十二周，肉眼观察，X射线和显微CT观察显示，新骨在质量，密度，硬度和结构上与正常骨非常接近。骨皮质光滑且与周围正常骨骼紧密相连。组织学观察发现修复区域有中度炎症，新的骨组织与正常组织相似。总之，本研究的组织工程骨表现出良好的骨传导性，可促进新的牙槽骨组织的形成，并且NELL1基因在骨再生中起促进作用。

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