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Identification and characterization of inorganic-phosphate-solubilizing bacteria from agricultural fields with a rapid isolation method

机译：快速分离法鉴定和鉴定农用增溶无机磷酸盐的细菌

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摘要

The ability to solubilize fixed inorganic phosphorus (P) for plant growth is important for increasing crop yield. More P can be released by inoculating soil with inorganic-phosphate-solubilizing bacteria (iPSBs). We used 96-well microplates instead of traditional 200-mm petri dishes to rapidly screen iPSB strains for their solubilizing ability. We simultaneously obtained 76 iPSB isolates from 576 wells containing two agricultural soils. This method conveniently identified positive iPSB strains and effectively prevented fungal cross-contamination. Maximum-likelihood phylogenetic trees of the isolated strains showed that Bacillus megaterium was the most dominant iPSB, and strains Y99, Y95, Y924 and Y1412 were selected as representatives for the analysis of P solubilization. Succinic acid was the main organic acid of B. megaterium for releasing P. It was strongly correlated with the increase in soluble P concentration during 168 h of incubation of these four strains. pH was negatively exponentially correlated with the amount of soluble P in the medium, and the amount of succinic acid was strongly linearly correlated with the amount of P released (P < 0.001), suggesting that organic acid may mobilize microbial P. Our study provides an efficient and effective method for identifying and analyzing the growth of iPSB strains able to solubilize inorganic P and gives a better understanding of the mechanism of P solubilization.
机译：溶解固定无机磷（P）以促进植物生长的能力对于提高农作物产量很重要。通过用增溶无机磷酸盐的细菌（iPSB）接种土壤可以释放更多的P。我们使用96孔微孔板代替了传统的200 mm培养皿，以快速筛选iPSB菌株的增溶能力。我们同时从包含两种农业土壤的576口井中获得了76种iPSB分离株。该方法可方便地鉴定出阳性iPSB菌株，并有效地防止了真菌的交叉污染。分离菌株的最大似然系统树表明，巨大芽孢杆菌是最主要的iPSB，并选择菌株Y99，Y95，Y924和Y1412作为P增溶分析的代表。琥珀酸是巨大芽孢杆菌释放P的主要有机酸。这与这四个菌株孵育168小时期间可溶性P浓度的增加密切相关。 pH值与培养基中可溶性P的含量呈负指数关系，琥珀酸的含量与P的释放量呈线性关系（P <0.001），这表明有机酸可以动员微生物P。鉴定和分析能够溶解无机P的iPSB菌株生长的高效有效方法，可以更好地理解P溶解的机理。

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