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首页> 美国卫生研究院文献>DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes >Comparative genomics of microsporidian genomes reveals a minimal non-coding RNA set and new insights for transcription in minimal eukaryotic genomes
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Comparative genomics of microsporidian genomes reveals a minimal non-coding RNA set and new insights for transcription in minimal eukaryotic genomes

机译：微孢子虫基因组的比较基因组学揭示了最小的非编码RNA集和最小的真核生物基因组转录的新见解

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摘要

Microsporidia are ubiquitous intracellular pathogens whose opportunistic nature led to their increased recognition with the rise of the AIDS pandemic. As the RNA world was largely unexplored in this parasitic lineage, we developed a dedicated in silico methodology to carry out exhaustive identification of ncRNAs across the Encephalitozoon and Nosema genera. Thus, the previously missing U1 small nuclear RNA (snRNA) and small nucleolar RNAs (snoRNAs) targeting only the LSU rRNA were highlighted and were further validated using 5' and 3'RACE-PCR experiments. Overall, the 15 ncRNAs that were found shared between Encephalitozoon and Nosema spp. may represent the minimal core set required for parasitic life. Interestingly, the systematic presence of a CCC- or GGG-like motif in 5' of all ncRNA and mRNA gene transcripts regardless of the RNA polymerase involved suggests that the RNA polymerase machineries in microsporidia species could use common factors. Our data provide additional insights in accordance with the simplification processes observed in these reduce genomes and underline the usefulness of sequencing closely related species to help identify highly divergent ncRNAs in these parasites.
机译：微孢子虫是普遍存在的细胞内病原体，其机会性性质导致其随着艾滋病大流行的增加而得到越来越多的认可。由于此寄生谱系在很大程度上未开发RNA世界，因此，我们开发了一种专门的计算机方法，可对整个Encephalitozoon和Nosema属的ncRNA进行详尽的鉴定。因此，突出显示了先前仅靶向LSU rRNA的U1小核RNA（snRNA）和小核仁RNA（snoRNA），并使用5'和3'RACE-PCR实验对其进行了进一步验证。总体而言，发现的15种ncRNA在Encephalitozoon和Nosema spp之间共有。可能代表寄生生命所需的最小核心集。有趣的是，无论涉及的RNA聚合酶如何，所有ncRNA和mRNA基因转录本的5'端都存在CCC样或GGG样基序，这表明小孢子虫中的RNA聚合酶机制可以利用共同因素。我们的数据根据​​在这些减少的基因组中观察到的简化过程提供了进一步的见解，并强调了对密切相关物种进行测序的有用性，以帮助鉴定这些寄生虫中高度不同的ncRNA。

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