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Construction of physical crosslink-based chitosan/liquid crystal composite hydrogel and evaluation on their cytocompatibility

机译:物理交联型壳聚糖/液晶复合水凝胶的构建及其细胞相容性评价

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摘要

In order to provide a novel biomimetic composite substrate for tissue engineering and explore the interaction between cells and this type of material, we developed chitosan/liquid crystal (CS/LC) composite hydrogel with embedded LC phases by composing of cholesterol hydroxypropyl cellulose ester liquid crystalline material and CS. The micromorphology of CS/LC composite hydrogels exhibited ‘islands-sea’ phase separation structures similar to the ‘fluid mosaic model’ of biomembrane. In vitro cell compatibility study suggested that 3T3 is fibroblasts exhibited better initial cell adhesions and higher proliferation rates on the composite hydrogel than on the polystyrene control plate and the pure LC membrane. This novel CS/LC composite hydrogel provides more favorable interface for cell growth and proliferation and may serve as potentially active substrate for engineering interfaces to live cells.
机译:为了提供一种新型的仿生复合材料用于组织工程,并探索细胞与此类材料之间的相互作用,我们通过组合胆固醇羟丙基纤维素酯液晶,开发了具有嵌入式LC相的壳聚糖/液晶(CS / LC)复合水凝胶。材料和CS。 CS / LC复合水凝胶的微观形态表现出类似于生物膜的“流体镶嵌模型”的“岛海相分离”结构。体外细胞相容性研究表明,与聚苯乙烯对照板和纯LC膜相比,复合水凝胶中的3T3成纤维细胞表现出更好的初始细胞粘附性和更高的增殖速率。这种新颖的CS / LC复合水凝胶为细胞的生长和增殖提供了更有利的界面,并且可以用作与活细胞工程化界面的潜在活性底物。

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